Homocysteine and Hypertension in Diabetes: Does PPARγ Have a Regulatory Role?

Figure 3

Increased media-lumen ratio of preglomerular arteriole and tubule of diabetic mice were normalized with ciglitazone treatment. Kidney sections of 0 wk (a), 10 wk of alloxan treatment (b), and 10 wk of alloxan treatment followed by another 6 wk of CZ treatment (c) were stained with Masson-Trichrome. (d) Preglomerular arterioles of these stained sections were identified under a microscope, and medial/lumen ratio was calculated by a digital micrometer and plotted ($\text{data}\mathrm{\hspace{0.17em}\hspace{0.17em}}\text{presented}\pm \text{SE}\text{,}$$n=6$ animals/group; ${}_{}{}^{*}P<.01$ compared with 0 wk; ${}_{}{}^{**}P\hspace{0.17em}<\hspace{0.17em}.05$ compared with 10 wk). The results indicated that medial/lumen ratio was increased dramatically due to thickening of the media and narrowing of the lumen after 10 wk of alloxan treatment. Interestingly, ciglitazone treatment almost normalized the media/lumen ratio indicating the involvement of PPAR$\gamma$ in this process.

(a)

(b)

(c)

(d)