Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver
Figure 1
Hepatic nuclear factor 4α(HNF4α) induced peroxisome proliferator-activated receptor γ (PPARγ) transcription activity in hepatocytes. (a) Quantitative RT-PCR (qRT-PCR) analysis of mRNA levels of PPARγ and its subtypes PPARγ1 and PPARγ2 in isolated primary hepatocytes. (b) HepG2 cells were cotransfected with plasmids of 3 × PPRE-Luc reporter plasmid with pMT7-HNF4α or vehicle plasmid for 24 hr, and luciferase activity with PPARγ antagonist BADGE and agonist rosiglitazone (Rosig) was measured. The β-gal plasmid was cotransfected as a transfection control. Promoter activities were measured by relative luciferase activity, which was normalized to that of β-gal. Results are mean ± SEM mRNA levels normalized to that of β-actin and expressed as fold of control group (DMSO). Results are representative of 3 independent experiments. *, **.
