Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver
Figure 2
HNF4α transactivated PPARγ2 promoter in hepatocytes. (a) Sequence of the 1.6-kb cloned human PPARγ2 promoter and sketch of putative HNF4α binding site. (b) HepG2 cells were infected with or without adenovirus HNF4α (Ad-HNF4α) for 24 hr and underwent chromatin immunopreciptitation (ChIP) assay with anti-HNF4α antibody; normal rabbit IgG was used in control experiments. qPCR was used with PPARγ promoter-specific primers to detect binding of HNF4α to the PPARγ2 promoter. The DNA levels were normalized to that of input and expressed as fold of the control group. *. (c) HepG2 cells were cotransfected with plasmids of PPARγ2p1505-Luc, PPARγ2p893-Luc, or PPARγ2p502-Luc with pMT7-HNF4α or vehicle plasmid. The β-gal plasmid was cotransfected as a transfection control. Promoter activities were measured by relative luciferase activity, with the level normalized to that of β-gal, from 3 independent experiments, each performed in triplicate. Data are mean ± SD. ** compared with control.
