Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver

Figure 3

Hepatic overexpression of HNF4α in rats decreased cholesterol levels in both plasma and liver. Rats were intravenously injected with Ad-HNF4α or Ad-GFP (𝑛=6), then killed 7 days post-infection. (a) Liver extracts were used to determine protein expression by western blot analysis with antibodies against HNF4α or β-actin. (b) Relative mRNA levels of hepatic HNF4α target genes, including G6p, Apoc3, Apob, and L-pk, were examined by qPCR. (c) Plasma cholesterol, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were determined. (d) Liver cholesterol was measured in lipid content of rat liver. Data are mean ± SEM, *𝑃<0.05, **𝑃<0.01 versus Ad-GFP infection.

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