171765.fig.005a
(a) Flank Xenograft
171765.fig.005b
(b) Thyroid Tumor
Figure 5: PPARγ knockdown attenuates both flank and thyroid orthotopic tumor growth of HTh74 anaplastic cancer cells. (a) 5 × 106 HTh74 cells stably expressing a scrambled (scr) control ( 𝑛 = 8 ) or PPARγ shRNA ( 𝑛 = 1 0 ) were injected sc into the right flank of an athymic nude mouse. Tumors were monitored visually and harvested after 8 weeks and measured in 3 dimensions with calipers. Final tumor volume was calculated with the following equation: volume (mm3) = L * W * D * 0.523. Mean ± SEM is reported (*, 𝑃 = 0 . 0 2 3 , two-tailed 𝑑 -test). (b) 0.5 × 106 HTh74 cells stably expressing a scrambled (scr) control ( 𝑛 = 9 ) or PPARγ shRNA ( 𝑛 = 8 ) were injected into the right thyroid lobe of an athymic nude mouse. Tumors were excised after 8 weeks and measured with calipers as before. The mean tumor volume ± SEM is reported (*, 𝑃 = 0 . 0 4 9 , two-tailed 𝑑 -test).