171765.fig.006a
(a)
171765.fig.006b
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171765.fig.006c
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Figure 6: Effect of PPARγ overexpression on the growth characteristics of BCPAP cells. (a) 40 μg of nuclear extract protein from BCPAP cells transfected with either pQCXIP (empty) or pQCXIP-expressing PPARγ was separated on a 10% SDS-PAGE gel, transferred to PVD, probed with PPARγ antibodies (sc-7196), and reprobed with PARP as described in Figure 2(a). (b) Cell growth rate was assessed by counting viable BCPAP cells transfected with pQCXIP (empty) or pQCXIP-expressing PPARγ at 2-day intervals. Data is represented as fold cell number over day 0 (5 expts. ± SEM; *, 𝑃 < 0 . 0 0 1 at day 6, 𝑃 < 0 . 0 0 0 1 at day 8 by 2-way ANOVA). (c) 50 μg of total cellular protein from BCPAP cells transfected with pQCXIP (empty) or pQCXIP-expressing PPARγ was separated on a 10% SDS-PAGE gel, transferred to PVD, and probed with antibodies against the corresponding cell cycle protein followed by the appropriate secondary antibody as described in Figure 4. β-actin was quantitated as a loading control.