Figure 7: Effect of PPARγ knockdown or overexpression on invasion. (a) HTh74 cells transduced with either scrambled (scr) or PPARγ-specific shRNA were serum starved (0.1% FBS) for 24 h. Cells (2 × 105) were transferred to a Boyden chamber and allowed to invade for 24 h toward medium containing 10% FBS. Cells on the bottom membrane were then stained with DAPI and counted using Metamorph Imaging Software (a representative DAPI-stained field is shown below the graph). Results shown are % inhibition of invading cells per field ± SEM (*, , ). (b) Empty vector or PPARγ-overexpressing BCPAP cells were subjected to invasion assays as described in (a) (*, , ).