Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice

Figure 3

Changes in hepatic expression of PPARs by fenofibrate treatment. Open and closed bars indicate mice treated without or with 0.1% fenofibrate, respectively. Data are expressed as mean ± SD (𝑛=6/group). *𝑃<0.05; **𝑃<0.01. (a) The mRNA levels of PPARs. Hepatic mRNA levels were normalized to those of GAPDH and then expressed as fold changes relative to those of Ppara (+/+) mice treated with a control diet. (b) PPAR-binding activity based on an enzyme-linked immunosorbent assay. Detailed protocols are described in Section 2. Results are expressed as fold changes relative to those of Ppara (+/+) mice treated with a control diet. (c) Immunoblot analysis. TBP was used as the loading control. Band intensities were measured densitometrically, normalized to those of TBP, and then expressed as fold changes relative to those of Ppara (+/+) mice treated with a control diet.

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