TGF1 Controls PPAR Expression, Transcriptional Potential, and Activity, in Part, through Smad3 Signaling in Murine Lung Fibroblasts
Figure 1
TGFβ1 modulates PPARγ expression, in part, via Smad3 signaling. (a) TGFβ1 and PPARγ mRNA expression. Murine primary lung fibroblasts were stimulated with TGFβ1 (10 ng/mL) for the indicated times. The treated cells were then submitted for analysis by RT-PCR for PPARγ. (b) Role of Smad3. Primary lung fibroblasts were isolated from the lungs of Smad3-deficient and wildtype mice and incubated with TGFβ1 (10 ng/mL) for 24 hours. Transcripts of PPARγ mRNA were detected using RT-PCR. *. (c) Regulation of PPARγ gene transcription. NIH/3T3 fibroblasts were transiently transfected with a luciferase reporter driven by the full-length PPARγ promoter and incubated with TGFβ1 (10 ng/mL). Cellular extracts were analyzed after 24 hours for luminescence. Luciferase activity was normalized to Renilla with data shown as fold change ± SE relative to control. *.
