Fenofibrate Enhances the In Vitro Differentiation of Foxp3⁺ Regulatory T Cells in Mice

Figure 1

Fenofibrate promoted the differentiation of Tregs in vitro. Total T cells (a) or CD4⁺T cells (d) were isolated from mouse spleens and induced to differentiate into Tregs with 5 ng/mL TGF-β, 50 U/mL IL-2, and fenofibrate doses indicated. The percentage of Foxp3⁺ T cells was analyzed by flow cytometry 4 days later. The mRNA level of Foxp3 in total T cells was analyzed by real-time PCR (b), and the percentages of Foxp3⁺ T cells in CD4⁺ and CD8⁺ T cell subgroups were analyzed by flow cytometry (c). In (a) and (d), numbers in the upper right corner indicate the final concentration of fenofibrate, and the outlined areas indicate Foxp3⁺ T cells with percentages shown in the lower right corner, 𝑛=5~6, *𝑃<0.05 versus 0 μM group. In (b) 𝑛=4, *𝑃<0.05versus 0 μM group. In (c) data represent 1 of at least 3 independent experiments.

(a)

(b)

(c)

(d)