In Vitro and In Vivo Characterizations of Chiglitazar, a Newly Identified PPAR Pan-Agonist
Figure 1
In vitro regulation of gene expression by chiglitazar. (a) Endogenous expression of individual PPAR subtypes in cell lines. (b) The indicated cell lines were treated with 10 μM rosiglitazone, 50 μM WY14643, 5 μM 2-bromohexadecanoic acid (2-Bro), 10 μM chiglitazar, or vehicle control (0.1% DMSO), respectively, for 48 h. Total RNA was isolated and RT-PCR was carried out using primers of the individual genes as listed in Table 1. The expression change of referred genes induced by different compounds was normalized against internal control (36B4 or GAPDH) and then compared with vehicle control treatment. The data shown are averaged from 3 independent experiments.
