Research Article

In Vitro and In Vivo Characterizations of Chiglitazar, a Newly Identified PPAR Pan-Agonist

Figure 3

In vivo regulation of gene expression by chiglitazar. (a) and (b) Normal male C57BL/6J mice (Nor) and male KKAy mice were orally administered daily with vehicle (Con), chiglitazar of 20 mg kg−1 (Chi), or rosiglitazone of 5 mg kg−1 (Ros) for 12 days. 24 h after the last dosing, tissues from abdominal fat, liver, and skeletal muscle were taken from 3 mice in each group, and total RNA was isolated from individual animal tissues. Equal amount of total RNA from each mouse in the same group was mixed and subjected to RT-PCR analysis for the indicated genes. (c) Male db/db mice were orally administered daily with vehicle (Con), rosiglitazone of 5 mg kg−1 (Ros), or chiglitazar of 20 mg kg−1 (Chi) for 14 days. 24 h after the last dosing, abdominal fat and skeletal muscle were taken from 3 mice in each group. Total RNA was isolated from individual animal tissues and subjected to the RT-PCR analysis independently for the indicated genes.
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