Pioglitazone Attenuates Vascular Fibrosis in Spontaneously Hypertensive Rats

<table>Effect of pioglitazone on mRNA and protein level of connective tissue growth factor (CTGF) and transforming growth factor <svg height="16.6625" id="M34" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><path d="M558 587q0 -32 -14 -61t-40 -53.5t-48.5 -41t-54.5 -36.5q144 -51 144 -174q0 -55 -43.5 -108t-104.5 -87q-77 -42 -131 -42q-31 0 -54 20t-31 47l11 18q48 -29 108 -29q79 0 119.5 43t40.5 109t-44.5 107.5t-119.5 50.5l22 47q34 1 65 21q96 61 96 157q0 42 -24 67.5
t-62 25.5q-24 0 -43.5 -9t-35 -29.5t-27 -44t-22.5 -63t-19.5 -75.5t-18.5 -91q-57 -294 -68 -380q-26 -190 -35 -200q-26 -31 -97 -37l-4 26q19 9 48 170l77 413q23 121 52.5 187.5t83.5 114.5q70 62 148 62q51 0 88.5 -34t37.5 -91z" id="x1D6FD"></path></g>
</svg> (TGF-<svg height="16.6625" id="M35" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><use xlink:href="#x1D6FD"></use></g>
</svg>) in rat aortas (a) Western blot analysis of protein level of CTGF and TGF-<svg height="16.6625" id="M36" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><use xlink:href="#x1D6FD"></use></g>
</svg>. <svg height="16.6625" id="M37" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><use xlink:href="#x1D6FD"></use></g>
</svg>-actin was an endogenous control.<svg height="17.825001" id="M38" style="vertical-align:-3.276pt" version="1.1" viewbox="0 0 80.487503 17.825001" width="80.487503" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,8.562,5.525)"><use xlink:href="#x2217"></use></g>
<g transform="matrix(.017,-0,0,-.017,16.175,14.875)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,37.363,13.688)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,52.066,13.688)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,60.226,13.688)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,64.102,13.688)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,72.261,13.688)"><use xlink:href="#x35"></use></g>
</svg> versus  WKY; <svg height="19.65" id="M39" style="vertical-align:-3.276pt" version="1.1" viewbox="0 0 75.887497 19.65" width="75.887497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,2.9,7.35)"><use xlink:href="#x23"></use></g>
<g transform="matrix(.017,-0,0,-.017,11.575,16.7)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,32.775,15.5)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,47.462,15.5)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,55.621,15.5)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,59.497,15.5)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,67.656,15.5)"><use xlink:href="#x35"></use></g>
</svg> versus SHR (b) Real-time RT-PCR analysis of mRNA level of CTGF and TGF-<svg height="16.6625" id="M40" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><use xlink:href="#x1D6FD"></use></g>
</svg>. Data are relative to GAPDH expression and are mean ± SD of 3 experiments. <svg height="17.825001" id="M41" style="vertical-align:-3.276pt" version="1.1" viewbox="0 0 74.8125 17.825001" width="74.8125" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,2.9,5.525)"><use xlink:href="#x2217"></use></g>
<g transform="matrix(.017,-0,0,-.017,10.5,14.875)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,31.7,13.688)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,46.387,13.688)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,54.546,13.688)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,58.422,13.688)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,66.581,13.688)"><use xlink:href="#x35"></use></g>
</svg> versus WKY; <svg height="19.65" id="M42" style="vertical-align:-3.276pt" version="1.1" viewbox="0 0 75.887497 19.65" width="75.887497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,2.9,7.35)"><use xlink:href="#x23"></use></g>
<g transform="matrix(.017,-0,0,-.017,11.575,16.7)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,32.775,15.5)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,47.462,15.5)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,55.621,15.5)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,59.497,15.5)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,67.656,15.5)"><use xlink:href="#x35"></use></g>
</svg> versus SHR.</table>

PPAR Research

fig5

Figure 5

Figure 5: Pioglitazone Attenuates Vascular Fibrosis in Spontaneously Hypertensive Rats 