Research Article
PPARγ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats
Table 3
Characteristics of primer pairs used, amplicon length, and efficiency of reaction in the RT-qPCR.
| |
Accession# | Gene | Primer sequence (5′ to 3′) | Product length (bp) | Efficiency |
| | JN236219.1 | ACACA | Forward: CTCCAACCTCAACCACTACGG
Reversal: GGGGAATCACAGAAGCAGCC | 171 | 2.09 | | JI861797.1 | AGPAT6 | Forward: AAGCAAGTTGCCCATCCTCA
Reversal: AAACTGTGGCTCCAATTTCGA | 101 | 2.17 | | X91503# | CD36 | Forward: GTACAGATGCAGCCTCATTTCC
Reversal: TGGACCTGCAAATATCAGAGGA | 81 | 2.18 | | DQ380249.1 | DGAT1 | Forward: CCACTGGGACCTGAGGTGTC
Reversal: GCATCACCACACACCAATTCA | 101 | 2.11 | | NM_001009350 | FABP3 | Forward: GATGAGACCACGGCAGATG
Reversal: GTCAACTATTTCCCGCACAAG | 120 | 2.14 | | DQ915966.3 | FASN | Forward: GGGCTCCACCACCGTGTTCCA
Reversal: GCTCTGCTGGGCCTGCAGCTG | 226 | 2.13 | | AJ431207 | GAPDH | Forward: GCAAGTTCCACGGCACAG
Reversal: GGTTCACGCCCATCACAA | 249 | 2.16 | | DQ997818 | LPL | Forward: AGGACACTTGCCACCTCATTC
Reversal: TTGGAGTCTGGTTCCCTCTTGTA | 169 | 2.18 | | GU332719 | NR1H3 | Forward: CATCAACCCCATCTTCGAGTT
Reversal: CAGGGCCTCCACATATGTGT | 163 | 2.13 | | HQ846826 | PLIN2 | Forward: TACGATGATACAGATGAATCCCAC
Reversal: CAGCATTGCGAAGCACAGAGT | 203 | 2.16 | | HQ846827 | PLIN3 | Forward: GGTGGAGGGTCAGGAGAAA
Reversal: TCACGGAACATGGCGAGT | 170 | 1.13 | | GQ918145 | PNPLA2 | Forward: GGAGCTTATCCAGGCCAATG
Reversal: TGCGGGCAGATGTCACTCT | 226 | 2.24 | | HQ589347.1 | PPARG | Forward: CCTTCACCACCGTTGACTTCT
Reversal: GATACAGGCTCCACTTTGATTGC | 145 | 2.21 | | DV935188# | SCAP | Forward: CCATGTGCACTTCAAGGAGGA
Reversal: TGTCGATCTTGCGTGTGGAG | 108 | 2.10 | | GU947654 | SCD | Forward: CCATCGCCTGTGGAGTCAC
Reversal: GTCGGATAAATCTAGCGTAGCA | 257 | 2.10 | | HM443643.1 | SREBF1 | Forward: CTGCTGACCGACATAGAAGACAT
Reversal: GTAGGGCGGGTCAAACAGG | 81 | 2.20 |
|
|
Annealing temperature for all primers in this table is 60°C.
ACACA, acetyl-coenzyme A carboxylase alpha; AGPAT6, 1-acylglycerol-3-phosphate O-acyltransferase 6; CD36, thrombospondin receptor; DGAT1, diacylglycerol acyl transferase 1; FABP3, fatty acid binding protein 3; FASN, fatty acid synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPL, Lipoprotein lipase; NR1H3, liver X receptor α; PLIN2, perilipin2; PLIN3, perilipin3; PNPLA2, patatin-like phospholipase domain containing 2; PPARG, peroxisome proliferator-activated receptor γ ; SCAP, cleavage activating protein; SCD, stearoyl-CoA desaturase; SREBF1, Sterol regulatory element-binding transcription factor 1.
#The primer sequences are from bovine.
|
