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PPAR Research
Volume 2013 (2013), Article ID 686525, 11 pages
http://dx.doi.org/10.1155/2013/686525
Review Article

Molecular Implications of the PPARs in the Diabetic Eye

Unidad de Investigación en Diabetes y Metabolism, CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Instituto de Investigación Vall d'Hebron, Universitat Autónoma de Barcelona, Paseo Vall d'Hebron, 119-129 Barcelona, Spain

Received 26 September 2012; Revised 7 January 2013; Accepted 7 January 2013

Academic Editor: Ruth Roberts

Copyright © 2013 Andreea Ciudin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetic retinopathy (DR) remains as the leading cause of blindness among working age individuals in developed countries. Current treatments for DR (laser photocoagulation, intravitreal corticosteroids, intravitreal anti-VEGF agents, and vitreoretinal surgery) are applicable only at advanced stages of the disease and are associated with significant adverse effects. Therefore, new pharmacological treatments for the early stages of the disease are needed. Emerging evidence indicates that peroxisome proliferator-activator receptors (PPARs) agonists (in particular PPARα) are useful for the treatment of DR. However, the underlying molecular mechanisms are far from being elucidated. This paper mainly focuses on PPARs expression in the diabetic eye, its molecular implications, and the effect of PPAR agonists as a new approach for the treatment of DR. The availability of this new strategy will not only be beneficial in treating DR but may also result in a shift towards treating earlier stages of diabetic retinopathy, thus easing the burden of this devastating disease (Cheung et al. (2010)).

1. Introduction

Diabetic retinopathy is the most common complication of diabetes, and proliferative diabetic retinopathy (PDR) remains the leading cause of blindness among working-age individuals in developed countries [1]. Diabetic macular edema (DME), another important event that occurs in diabetic retinopathy, is more frequent in type 2 than type 1 diabetes [2]. Although PDR is the most common sight-threatening lesion in type 1 diabetes, DME is the primary cause of poor visual acuity in type 2 diabetes. Because of the high prevalence of type 2 diabetes, DME is the main cause of visual impairment for diabetic patients. In addition, DME is almost invariably present when PDR is detected in type 2 diabetes [3]. Despite heterogeneity in patient selection criteria, country and selection period, the prevalence of patients with DR in Western countries is relatively similar, ranging from 21.9 to 36.8% [4]. Population-based studies suggest that about one-third of the diabetic population have signs of DR and one-tenth have vision-threatening states of retinopathy such as diabetic maculae edema (DME) and proliferative diabetic retinopathy (PDR) [5, 6].

Neovascularization caused by severe hypoxia is the hallmark of PDR, whereas vascular leakage caused by the breakdown of the blood retinal barrier (BRB) is the main event involved in the pathogenesis of DME.

Healthcare costs for patients with DR are almost double than that of patients without it and they increase considerably with the severity of DR [7, 8], which suggests that preventing the progression of DR may significantly reduce the economic burden related to this complication of diabetes [9].

Current treatments for DR (laser photocoagulation, intravitreal corticosteroids, intravitreal anti-VEGF agents, and vitreoretinal surgery) are applicable only at advanced stages of the disease and are associated with significant adverse effects [10, 11]. Therefore, new pharmacological treatments for the early stages of the disease are needed.

In recent years, several experimental and clinical studies have shown the beneficial effects of peroxisome proliferator-activator receptors (PPARs) agonists (in particular PPARα) in diabetic retinopathy. However, the molecular mechanisms are far from being elucidated. In this paper, we review PPARs expression in the diabetic eye, its molecular implications, and the effect of PPAR agonists as a new approach for the treatment of DR.

2. Peroxisome Proliferator-Activator Receptors (PPARs) and Their Agonists: A General Overview

Peroxisome proliferator-activator receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that regulate gene expression in response to nutritional and physiological stimuli.

The nuclear receptor superfamily can be divided into two categories: first, the classic hormone receptors that bind specific hormones (glucocorticoids, thyroid hormones, and estrogen) and second, nuclear receptors that act as metabolic sensors, binding to substrate or end-products of metabolic pathways, such as the liver X receptors, the farnesoid X receptor, hepatocyte nuclear factor 4α, or PPARs [12].

PPARs were initially identified as a peroxisome proliferator “binding-protein” capable of inducing hepatocyte peroxisome proliferation. Peroxisomes are subcellular organelles whose main function is the removal of molecular oxygen and breaking down hydrogen peroxide [13, 14]. However, the peroxisomes are also involved in glycerolipid synthesis, fatty-acid oxidation, glucose and cholesterol biosynthesis and metabolism [15].

The PPARs function together with the retinoid X receptor (RXR) is to regulate glucose and lipid metabolism. In the presence of the specific ligands, the PPARs adopt an active conformation by forming a heterodimer with the RXR, resulting in binding to peroxisome proliferator response elements in target genes, determining PPAR-dependent gene expression [16]. A PPAR is a compact molecule, consisting of 5 or 6 structural regions (A–F) divided into four functional domains. The C-domain of the PPARs is DNA-binding and the E or F region is the ligand-binding site. The ligand-binding site has a pivotal role in transcriptional activation [15].

Despite the high levels of homologies at the protein level, three isoforms have been identified, each of which has different numbers of amin oacid residues: PAARα (468 amino acids residues), PAAR β/δ (441 amino acids), and PPARγ (479 amino acids) [17]. They are distributed differently in the body tissues and exert different functions.

PPARα is highly expressed in tissues with elevated mitochondrial and peroxisomal fatty-acid beta-oxidation rates, such as liver, heart muscle, kidney, skeletal muscle, brown fat, and retina [1820]. PPARα is also present in monocytes, macrophages, and endothelial cells [21].

PPAR β/δ are distributed ubiquitously in almost all tissues and recent data suggests their involvement in cell proliferation, angiogenesis, and inflammation [22]. PPARγ has been intensively studied for its crucial implication in glucose homeostasis and insulin sensitivity [23]. PPARγ is also involved in the regulation of lipid metabolism by increasing the genes that regulate fatty-acid uptake and storage [24] and plays a pivotal role in adipocyte differentiation and function [25].

So far seven PPARγ isoforms have been identified, most of them (PPARγ1, PPARγ2, PPARγ3, PPARγ6, and PPARγ7) expressed abundantly in adipose tissue. PPARγ4 and PPARγ5 are expressed only in macrophages [23].

2.1. PPARα Agonists

The first PPAR discovered was PPARα, during the search of a molecular target for a group of agents then referred to as peroxisome proliferators, as they increased peroxisomal numbers in rodent liver tissue, apart from improving insulin sensitivity. These receptors, pharmacologically related to the fibrates, were discovered in the early 1980s [26]. The main natural ligands of PPARα are the fatty acids and endogenous eicosanoids. Physiological concentrations of diet-derived unsaturated fatty acids also activate PPARα [19]. In addition, PPARα are strongly stimulated by synthetic molecules such as the fibrates, a class of amphipathic carboxylic acids (gemfibrozile, clofibrate and fenofibrate). The main effects of PPARα-stimulation in humans are decreasing triglycerides, shifting low-density lipoprotein cholesterol to larger particles, and increasing high-density lipoprotein cholesterol particles.

2.2. PPARγ Agonists

The main natural ligands of PPARγ are the fatty acids, the phospholipids and their oxidatively modified metabolites, and a group of natural nitroalkenes [27]. One of the natural ligands of PPARγ is an oxidatively modified phospholipid, 15-deoxy-δ 12,14-prostaglandin J2 (15d-PGJ2) and there are also other J2 series prostaglandins. In general, the endogenous ligands have a relatively low affinity and show little specificity towards the different PPARs. Additionally, most of the effects of PGJ2 occur independent of PPARγ.

In 1995, a class of antidiabetic drugs, the thiazolidinediones (TZDs), were shown to activate PPARγ with high affinity, even more effectively than any natural ligand [28]. Various synthetic ligands for PPARγ were developed, such as the TZD family: ciglitazone, troglitazone, rosiglitazone, and pioglitazone. Some non-TZD synthetic ligands such as GW1929 and GW7845 are mainly used in experimental research.

2.3. Dual α/γ Agonists

In order to combine the beneficial effects of both PPARα and PPARγ activation, dual α/γ agonists have recently been developed. Synthetic molecules, like muraglitazar or tesaglitazar, were shown to be superior to TZDs in terms of improving glucose metabolism and raising HDL-c levels in T2D patients, but the phase III clinical trials have been discontinued [29]. A meta-analysis of the phase II and III clinical trials of muraglitazar revealed that it was associated with a greater incidence of myocardial infarction, stroke, transient ischemic attacks, and CHF when compared to placebo or pioglitazone [30]. Tesaglitazar was generally well tolerated but was associated with a greater increase in serum creatinine level than placebo [31]. At present, aleglitazar, a dual PPAR α/γ agonist, currently in phase III clinical development, seems to be a potent and balanced activator of PPARα and γ, with beneficial effects in type 2 diabetic patients who have suffered a recent cardiovascular event [32, 33].

Telmisartan, an angiotensin-II receptor (AT-1) blocker, has been proven to be a dual PPAR δ/γ agonist, and bezafibrate is a pan-PPAR agonist. Furthermore, telmisartan was shown to have a beneficial effect in a murine model of retinal neovascularization, by promoting a dual AT-1 blockade and PPARγ activation, suggesting a possible implication in the treatment of DR [34, 35].

Several angiotensin-II receptor blockers (candesartan, irbesartan, losartan, but not valsartan or olmesartan) were shown to activate PPARγ ligands in vitro. However, only telmisartan, and to a lesser extent candesartan, resulted in significant PPARγ agonism in cell cultures, but in vivo, sartan treatment had no effect as insulin sensitizers [36] suggesting a very weak PPARγ stimulation in vivo.

2.4. Dual α/δ Agonists

Molecules targeting both PPARα and PPARδ were also developed. GFT505 is a dual α/δ agonist currently completing a phase II clinical trial. It shows a good tolerance and a significant improvement of lipid and glucose disorders associated with metabolic syndrome [37].

3. Distribution of the PPARs in the Retina

All of the PPARs are constitutively expressed in the whole retina [38] but most of our knowledge refers to the retinal pigment epithelia (RPE). It has recently been shown that PPARs are expressed in cultures of primary RPE cells and ARPE19 cells (a human immortalized line of RPE cells) [39]. Both cell types presented a lack of PPARγ2 and moderate PPARγ1 and PPAR β/δ expression. The ARPE-19 cells showed a moderate expression of PPARα while primary RPE cells had a low representation. Interestingly, the same study analyzed the PPARs in the freshly isolated RPE, and the results were slightly different from the studies of cell cultures: a lack of PPARγ1 and PPARγ2 expression and a high representation of PPARα and PPAR β/δ. However, the results of the freshly isolated RPE might have been altered due to the small number and the significant heterogeneity of the sample and are in contrast to the findings of previous studies which demonstrated the presence of PPARγ in the RPE [40]. These conflicting results suggest that further studies addressed to evaluating the distribution of the PPARs not only in RPE, but also in the neuroretina are needed.

4. Molecular Implications of PPARγ Activation in Diabetic Retinopathy

PPARγ activation plays a key role in the development of DR. In fact, several experimental studies have shown that PPARγ receptors are downregulated in the diabetic eye and that their suppression is involved in the pathogenesis of DR [41, 42]. In addition, there is clinical evidence to suggest that some PPARγ polymorphisms represent a genetic risk factor for developing DR [43, 44].

PPARγ activation might participate in abrogating the two most important events that occur in DR: neurodegeneration and microangioapathy.

4.1. Effect of PPARγ Activation in Retinal Neurodegeneration

Although DR has been classically considered to be a microcirculatory disease of the retina, there is emerging evidence to suggest that retinal neurodegeneration is an early event in the pathogenesis of DR which antedates and participates in the microcirculatory abnormalities that occur in DR [45, 46]. In this regard, it is worth mentioning that the main features of retinal neurodegeneration (apoptosis and glial activation) have been found in the retinas of diabetic donors without any microcirculatory abnormalities appearing in the ophthalmoscopic examinations performed during the year before death [47, 48]. Retinal ganglions cells (RGCs), located in the inner retina, are the retinal neurons in which the apoptosis process related to diabetes is first detected [49]. Regarding glial activation, it is important to note that a complex milieu of dysregulated proinflammatory factors occurs in the diabetic retina, and while retinal microglia and infiltrating monocytic cells probably make an important contribution, there is also strong evidence that Müller glia shows inflammation-linked responses when exposed to the diabetic milieu [50, 51].

Glutamate, the major excitatory neurotransmitter in the retina is an essential mediator of the retinal neurodegenerative process that occurs in DR. Glutamate has been found elevated in the extracellular space in experimental models of diabetes [5255]. This extracellular and synaptic excess of glutamate leads to the overactivation of the ionotropic glutamate receptors, mainly alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) and N-methyl-D-aspartame (NMDA) receptors, which results in an uncontrolled intracellular calcium response in postsynaptic neurons and cell death [56, 57]. This deleterious effect of glutamate on retinal neurons is known as “excitotoxicity” and retinal ganglion cells are primarily affected.

Aoun et al. [58] demonstrated that two PPARγ ligands, 15d-PGJ2 and troglitazone protect RGC-5 (an established transformed rat retinal ganglion cell line) against glutamate insult. The neuroprotective effects of the two compounds appeared to be mediated through an antioxidant rather than a PPAR-gamma-dependent pathway. In addition, PPARγ is constitutively expressed in rat primary microglial cultures and PPARγ activation had an anti-inflammatory effect [59, 60]. Furthermore, it has recently been demonstrated that rosiglitazone attenuates diabetes-induced apoptosis in retinal neurons of STZ-induced diabetic rats by inhibition of phospho-STAT3 (p-STAT3) and cytokine signaling 3 (SOCS3) [61].

Apart from the neuroprotection due to the abrogation of excitotoxicity, there is emerging evidence that PPARγ activation also inhibits the neurotoxicity resulting from the overexpression of the renin-angiotensin system that exists in DR [6265].

Finally, activation of PPARgamma may play an important role in regulating the expression of target genes that are involved in lipid and fatty acid metabolism in the photoreceptor renewal process. The RPE is a specialized epithelium lying in the interface between the neural retina and the choriocapillaris where it forms the outer blood-retinal barrier (BRB). However, the RPE is something more than a constituent of the BRB and the phagocytosis of shed photoreceptor membranes is vitally important for maintaining the structural and functional integrity of the retina [66]. In fact, photoreceptor homeostasis is maintained mainly by the RPE’s phagocytic capacity, which is able to degrade between 25.000 and 30.000 outer segment disks daily [67]. The RPE plays a central role in the processing and recycling of fatty acids and other lipids resulting from photoreceptor digestion. Ershov et al., using primary cultures from rat RPE cells, demonstrated that photoreceptor phagocytosis selectively activates PPARγ expression while having no effect on PPARα or PPAR β/δ [67]. This result suggests the implication of PPARγ in preventing the possible deleterious effect of fatty acid accumulation in the retina, thus conferring neuroprotection.

4.2. Effect of PPARγ Activation on Microangiopathy

Apart from neuroprotection, PPARγ activation has beneficial effects on early microvascular abnormalities. The loss of pericytes and endothelial dysfunction are the hallmarks of these early stages of diabetic microangiopathy, and the balance between nitric oxide (NO) [vasodilator] and endothelin-1 (ET-1) [vasoconstrictor] is essential in determining the hemodynamic response of the capillaries. One of the most important functional impairments in the early stages of DR is the reduced production and bioavailability of NO [68] and the increase of ET-1 [69]. A study focused on NO production in pericytes showed that PPARγ is constitutively expressed in the retinal pericytes and that troglitazone increased NO production in a PPARγ-dependent manner [70]. In addition, it has been shown that pioglitazone elicits the endothelium-dependent dilation of isolated porcine retinal arterioles mediated by NO release [71]. Furthermore, PPARγ activation inhibits ET-1 secretion from endothelial cells [72]. Taken together, PPARγ activation exerts a clear beneficial effect on the imbalance between NO and ET-1 that exists in DR.

VEGF plays an essential role in the pathogenesis of DR by leading to the disruption of the BRB (the main pathogenic event in the development of DME) and by promoting neovascularisation (the hallmark of PDR) [73]. Therefore, the effect of PPARγ agonists on VEGF plays a key role in accounting for the clinical and experimental results of PPARγ agonists, in particular TZDs in the development of both DME and PDR.

The relationship between TZDs and DME has generated an intense debate that is still far from being elucidated. Clinical evidence indicates that TZDs significantly increase the risk of heart failure by a PPARγ-mediated mechanism which leads to fluid retention. Because of this effect, there has been concern about the possible relation between the use of TZDs and DME. There are several studies which support this theory, especially in patients having impaired renal function, cardiac failure, or associated insulin therapy [7478]. Fong and Contreras [77] in a large prospective cohort study showed that TZDs users were more likely to develop DME (OR, 2.6 [95% CI, 2.4–3.0]). Idris et al. [78], in a retrospective cohort study of 103,368 patients with type 2 diabetes mellitus and without DME at baseline, showed at 1 year, an incidence of DME of 1.3% ( ) and 0.2% ( ) among thiazolidinedione users ( ) and nonusers ( ), respectively. After Cox multiple regression analysis, multiple imputation analysis to adjust for missing values, and propensity score analysis to exclude any selection bias, TZD use was associated with an increased risk of DME at 1-year followup (OR, 2.3 [95% CI, 1.5–3.6]) and 10-year followup (HR, 2.3 [95% CI, 1.7–3.0]). The effect was similar for pioglitazone and rosiglitazone. Combination therapy with insulin plus TZD was associated with a higher risk of DME after propensity score adjustment (HR, 3.0 [95% CI, 1.5–5.9]). By contrast in the ACCORD-eye substudy, no association was observed between TZD exposure and DME in patients with type 2 diabetes [79]. In this study, the cross-sectional association of DME and visual acuity with TZD was examined by means of baseline fundus photographs and visual acuity measurements from the ACCORD trial. TZD use was not associated with DME in unadjusted (odds ratio [OR], 1.01; 95% confidence interval [CI], 0.71–1.44; ) and adjusted (OR, 0.97; 95% CI, 0.67–1.40; ) analyses. In addition, TZD use was associated with slightly greater visual acuity (0.79 letter; 95% CI, 0.20–1.38; ) but this effect was of uncertain clinical significance. Nevertheless, it is worthy of mention that the ACCORD-EYE study had an important selection bias because patients at potential risk for macular edema were excluded [80]. There is little experimental information on the relationship between PPARγ activation and DME development. Muranaka et al. [81] demonstrated that rosiglitazone was effective in protecting against the breakdown of the BRB in streptozotocin-induced diabetic rats but this effect was not mediated by VEGF downregulation. By contrast, Zheng at al. [82] have recently found that simvastatin decreases retinal vascular permeability in streptozotocin-induced diabetic rats through the inhibition of VEGF expression and p38MPAK activity mediated by the PGC-1α. It was demonstrated that PPARγ activation by PIO upregulates PGC-1α suggesting a protective effect of [83] PPARγ activation on the breakdown of the BRB induced by diabetes. Taken together, current evidence regarding the relationship between PPARγ activation and BRB function is confusing and further clinical and experimental studies addressed to examining this issue are urgently required.

The role of PPARγ activation in VEGF-induced PDR is also worthy of discussion. Several studies showed a proangiogenic effect of PPARγ activation by the increased expression of VEGF, suggesting a possible deleterious effect in PDR [8486]. However, these studies were performed in human vascular muscle cell [84], adipocytes [85], and cultured cardiac myofibroblasts [86]. The heterogeneity of the behaviour of endothelial cells in several body compartments is well known. Therefore, the results obtained from a certain cell type cannot be extrapolated to another. In the retina, it seems that PPARγ activation has an antiangiogenic effect. Murata et al. [87] using the oxygen-induced ischemia (OII) mouse model of retinal neovascularization showed that PPARγ activation by intravitreous administration of troglitazone or rosiglitazone inhibited VEGF-mediated neovascularisation. Interestingly, VEGF was not significantly inhibited in the ganglion cell layer, thus preserving the neuroprotective properties of VEGF in this critical neural layer. Aljada et al. [88] demonstrated by using chick chorioallantoic membrane (CAM) model that rosiglitazone and pioglitazone inhibited the proangiogenic effects of bFGF (basic fibroblast growth factor) and VEGF. Higuchi et al. [89] found that pioglitazone attenuated pathological retinal microvessel formation in a mouse model of OII through adiponectin-mediated modulation of TNFα production. More recently, Rodrigues et al. [90] using ARPE-19 cell cultures demonstrated that PPARγ agonists can have differential effects on RPE survival in response to oxidative stress: troglitazone but not rosiglitazone or pioglitazone was able to improve the RPE response to oxidative stress by downregulating VEGF expression. Finally, Hatanaka et al. [91], have shown that pioglitazone inhibits fibrotic change in primary monkey RPECs through the suppression of TGF-β signaling. A clinical study which supports these experimental findings was conducted by Shen et al., [92] who showed in a case-control study that rosiglitazone reduced the progression from NPDR to PDR over 3 years by 59%.

Apart from the potential beneficial effects on neurodegeneration and microvascular abnormalities, PPARγ activation might also counteract other mechanisms involved in the pathogenesis of DR such as inflammation and leukostasis [81, 93, 94], the overexpression of matrix metalloproteinases [95] or the increase in platelet aggregation [96].

5. Molecular Implications of the PPARα in Diabetic Retinopathy

Ever since 1969 there has been clinical evidence of a beneficial effect of PPARα activation on diabetic retinopathy. Harrold et al. showed an improvement in retinal exudate after 1 year of treatment with clofibrate, without significant effects on other retinal lesions [97]. The data was confirmed by Dorne in 1977, who suggested clofibrate as the treatment of choice for exudative diabetic retinopathy [98].

The current evidence that PPARα activation has a beneficial effect in DR comes from two seminal clinical trials: the FIELD [99, 100] and the ACCORD-Eye [101] studies which showed that DR progression was significantly reduced by fenofibrate (a PPARα used as a hypolipemiant agent).

The FIELD Study raised the prospect of the prevention of DR via treatment with fenofibrate. FIELD was essentially a cardiovascular trial, with a large population ( ) of type 2 diabetes patients without statin treatment at baseline randomised to receive fenofibrate or placebo for 5 years [99]. Primary and secondary endpoints focussed on cardiovascular events. Eight per cent of the population of FIELD had retinopathy at baseline, and the need for laser photocoagulation for retinopathy was included among the a priori tertiary endpoints of the trial [99]. Fenofibrate reduced the incidence of patients requiring laser photocoagulation (from 5.2% on placebo to 3.6%, ). There was greater absolute benefit in patients with, rather than without, pre-existing retinopathy [100]. However, baseline photographic assessment of retinal status was only made in 10% of patients. Moreover, the criteria for the use of laser treatment were not pre-specified and therefore are likely to have been heterogeneous [102].

A substudy conducted in 1012 patients explored the effects on retinopathy outcomes in the FIELD study in more detail [100]. In this ophthalmological substudy, retinopathy status and severity were assessed from two-field 45° colour fundus photographs of the macula (stereoscopic) and a disc/nasal field taken at baseline, 2 years and 5 years, and graded with Early Treatment Diabetic Retinopathy Study (ETDRS) criteria. A marked and significant reduction (~70%) in the risk of laser treatment for retinopathy was again demonstrated for fenofibrate versus placebo. However, only 28 patients required laser treatment (23 in the placebo group and 5 in the fenofibrate group). In addition, DR progression, (2-step Early Treatment Diabetic Retinopathy Study [ETDRS] scale, the primary endpoint), was significantly reduced with fenofibrate in those patients with preexisting DR at baseline (from 14.6% to 3.1%, ), but not in those without DR at baseline. However, the number of events was small (14 in the placebo group and 3 in the fenofibrate group).

The ACCORD trial included a lipid arm, in which patients were randomly assigned to treatment with fenofibrate or placebo in addition to open-label simvastatin [103]. Patients eligible for this arm were also enrolled in the glycemia evaluation, but met additional recruitment criteria relating to lipids (LDL cholesterol 1.55–4.65 mmol/L, HDL cholesterol <1.29 mmol/L [<1.42 mmol/L for women], and triglycerides <8.5 mmol/L [<4.5 mmol/L if receiving lipid-modifying therapy]). Retinopathy outcomes in ACCORD were evaluated in a 4-year eye substudy [101]. Randomization to fenofibrate relative to placebo (on background therapy with simvastatin) was associated with a significant decrease (from 10.2% to 6.5%, ) in DR progression (3 or more steps on the EDTRS), with greater effect in patients with evidence of DR at baseline (absolute RR 6.9% versus 0.2% in those without DR at baseline). It should be noted that the reduction obtained with fenofibrate plus simvastatin (−40%; ) was even higher than that obtained in the arm of intensive glycaemic control (−33%; ).

The similarities between the effects of fenofibrate on outcomes in the eye between the FIELD and ACCORD-EYE studies are striking. In both trials, randomization to fenofibrate (in combination with a statin in a substantial proportion of patients by study end in FIELD, and exclusively with a statin in ACCORD-EYE) led to statistically and clinically significant reductions in the risk of a range of clinical endpoints related to retinopathy [99101]. In summary, these trial data show that fenofibrate treatment provides a relative reduction in DR progression of 30–40% over 4 to 6 years, with greater benefit in patients with pre-existing DR. These benefits were achieved despite a lack of significant reductions in the risk of the primary composite cardiovascular endpoint in either study (although there was a significant reduction in total cardiovascular events in FIELD) [99, 103].

5.1. Mechanisms of Action of Fenofibrate
5.1.1. Lipid-Mediated Mechanisms

Fenofibrate is indicated for the treatment of hypertriglyceridemia and mixed dislipidemia. Its main action is to lower plasma triglyceride levels, but it also reduces total and LDL cholesterol, raises HDL cholesterol, and decreases the concentration of small LDL cholesterol particles and apolipoprotein B. In the FIELD study, there was essentially no change in HDL-cholesterol with fenofibrate (mean reduction of 0.01 mmol/L for fenofibrate versus placebo at study end) and only a modest change in triglycerides (mean reduction 0.24 mmol/L) [99]. In ACCORD, mean HDL-cholesterol increased from 0.98 mmol/L to 1.07 mmol/L with fenofibrate and from 0.99 mmol/L to 1.05 mmol/L with placebo [103]. Similarly, baseline and final triglyceride levels were 1.85 mmol/L and 1.38 mmol/L in the fenofibrate group of the ACCORD lipid arm, compared with 1.81 mmol/L and 1.63 mmol/L in the placebo group. Therefore, it seems that the beneficial effects of fenofibrate on diabetic retinopathy are unrelated to quantitative changes of serum lipids. However, it is not known whether the effectiveness of fenofibrate in modulating the qualitative properties of lipoproteins (i.e., reducing remnants and small dense LDL particles) can contribute to its beneficial effects.

It should also be noted that the mechanisms regulating intraretinal lipid transport, rather than serum lipid levels, might be more important in the pathogenesis of DR [102]. In this regard, we have recently shown that apolipoprotein A1 (apo-A1) is overexpressed in the retina of diabetic patients [104, 105]. Apo-A1 is a key factor for the intraretinal transport of lipids, thus preventing lipid deposition and lipotoxicity, and is also a potent scavenger of reactive oxygen species. Therefore, apo-A1 could play an important role in protecting the retina from oxidative stress. These findings have led us to hypothesize that the retinas of diabetic patients have a higher content of apo-A1 as a protective mechanism and, consequently, that those patients with less capacity for apo-A1 production by the retina will be more prone to develop lipid deposition (hard exudates) and retinal damage induced by oxidative stress. Fenofibric acid has been shown to enhance transcription of the apoA-1 gene in the liver [106], macrophages, and fibroblasts [107], but whether this is also true at the retinal level remains to be elucidated.

Finally, it has recently been shown that circulating apoAI may be an independent protective factor for the development of DR [108]. Therefore it is possible that the increase in apoAI plasma levels induced by fenofibrate participates in its beneficial action on DR.

5.1.2. Nonlipidic-Mediated Mechanisms

There are several nonlipidic mechanisms by which fenofibrate, or its active metabolite, fenofibric acid (FA) can exert beneficial effects in preventing or arresting DR.

5.2. Neuroprotective Effect

As previously mentioned, neurodegeneration plays an essential role in the pathogenesis of DR. In experimental models of cerebral ischaemia and neurodegenerative diseases, PPARα activation had a neuroprotective effect, independent of lipid metabolism [109]. Antioxidant, anti-inflammatory, and antiapoptotic properties of fenofibrate have been implicated in this effect.

5.3. Improvement in Endothelial Function and Anti-Apoptotic Activity

FA exerts a protective effect on the microvasculature by suppressing apoptosis and stimulating nitric oxide synthase (eNOS) phosphorylation and NO production. This is mediated by AMPK activation, as has been shown by studies of numerous cell systems, including human retinal endothelial cells [110113]. This effect is unrelated to PPARα activation, as is evident in human retinal endothelial cells [113]. In addition, a recent study showed that FA elicits dual protective effects in the RPE by the down-regulation of stress-mediated signalling and the induction of autophagy and survival pathways [114].

5.4. Antioxidant and Anti-Inflammatory Activity

Fenofibrate may mitigate the adverse effects of oxidative and inflammatory stress, which are involved in the development of DR. It has been reported that PPARα activation induces the expression and activation of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase [109], thus ameliorating oxidative stress, a key factor for the development of DR [115].

PPARα activation induces the apoptosis of human monocyte-derived macrophages [116] and inhibits the expression of vascular cell adhesion molecules on the endothelium [117]. These effects might be potentially relevant in preventing leukostasis. Furthermore, it has recently been shown that FA prevents the deleterious action of IL-1β in the disruption of the BRB, thus supporting the role of proinflammatory cytokines in the pathogenesis of DME [118].

5.5. Preventive Effects on Blood-Retinal Barrier Breakdown

The breakdown of the BRB, due to the disruption of tight junctions with subsequent leakage, is the main factor implicated in DME. Fenofibrate prevents DME progression, suggesting a possible effect in reducing the permeability associated with DR. In cultures of human RPE cells (which constitute the external BRB), FA prevented the disorganisation of tight junction proteins and hyperpermeability provoked by the diabetic milieu. This is mediated by the effect of FA in reducing interleukin-β-induced AMPK activation [118]. Finally, it has recently been shown that FA down-regulates the overexpression of basement membrane components (fibronectin and collagen IV) in RPE cells cultured in conditions mimicking the diabetic milieu. Exposure to FA reduced the increase in permeability associated with the overexpression of fibronectin and collagen IV in a dose-dependent manner [119].

5.6. Antiangiogenic Activity

PPAR-α is present in endothelial cells [120], and its activation by means of PPAR-α agonists has recently been shown to inhibit expression of VEGF receptor 2 (VEGFR2) and neovascularization in human umbilical endothelial cells [121]. Varet et al. [122] have demonstrated that fenofibrate inhibits angiogenesis in vitro and in vivo as well as basic fibroblast growth factor-induced angiogenesis in vivo. In addition, in cells derived from human ovarian cancer, clofibric acid (a PPAR-α agonist) downregulates VEGF expression [123]. Finally. Chen et al. [124] have recently shown that both oral and intravitreal administration of fenofibrate ameliorated leukostasis and retinal vascular leakage in type 1 murine models, and that they also attenuated the overexpression of adhesion molecules and VEGF. The beneficial effects of fenofibrate were blocked by a specific PPARα antagonist, thus suggesting a PPARα-dependent mechanism.

6. Molecular Implications of the PPAR β/δ in Diabetic Retinopathy

PPAR β/δ is also expressed in the retina. Little is known about the effects of PPAR β/δ in the eye and less about its effects in the diabetic eye. Most of the studies have associated PPAR β/δ activation with proangiogenic and proinflammatory effects. The PPAR β/δ agonist GW501516 stimulates human umbilical vein endothelial cells proliferation and increased VEGF expression [125].

7. Conclusions

Improvements in diabetes care and management have been crucial in lowering the incidence and severity of DR. Nevertheless, DR remains the most common cause of vision impairment in working age adults in the US and Europe and retinal neovascularization occurs in up to 20% of patients with diabetes. As greater knowledge of the molecular mechanisms involved in the pathogenesis of DR has been obtained, new therapeutic products have been developed. In this regard, scientific evidence has accumulated in recent years regarding the role of PPAR activation in the pathogenesis of DR. As far as we know, PPARγ activation would appear to have a beneficial effect in the early stages of DR. The beneficial effects of PPARα activation by fenofibrate in DR have been demonstrated in two large clinical trials (FIELD and ACCORD-EYE) with greater benefit in patients with pre-existing DR. By contrast, PPAR β/δ activation has a deleterious effect, promoting inflammation and angiogenesis. However, the underlying mechanisms by which PPARs are exerting their effects in the retina are only just beginning to be understood.

Concerted efforts to better define the presence and distribution of PPARs in freshly isolated human retina and specific studies addressed to examining the mechanistic pathways and functional effects involved in PPAR activation in both nondiabetic and diabetic retina are urgently needed.

Finally, improved understanding of the mechanisms of action of PPARs will facilitate their clinical application. Ophthalmologists and physicians treating diabetic patients should be aware of the potential usefulness of PPARs and work together not only in future research, but also in establishing clinical guidelines that will include these drugs as medical treatments for DR. Only such coordinated action, together with rational strategies targeting prevention, will be effective in reducing the burden of DR and improving clinical outcomes related to this devastating complication of diabetes.

References

  1. N. Cheung, P. Mitchell, and T. Y. Wong, “Diabetic retinopathy,” The Lancet, vol. 376, no. 9735, pp. 124–136, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Klein, S. E. Moss, B. E. K. Klein, M. D. Davis, and D. L. DeMets, “Wisconsin epidemiologic study of diabetic retinopathy. XII. Relationship of C-peptide and diabetic retinopathy,” Diabetes, vol. 39, no. 11, pp. 1445–1450, 1990. View at Scopus
  3. L. Tong, S. A. Vernon, W. Kiel, V. Sung, and G. M. Orr, “Association of macular involvement with proliferative retinopathy in type 2 diabetes,” Diabetic Medicine, vol. 18, no. 5, pp. 388–394, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. C. Delcourt, P. Massin, and M. Rosilio, “Epidemiology of diabetic retinopathy: expected vs reported prevalence of cases in the French population,” Diabetes and Metabolism, vol. 35, no. 6, pp. 431–438, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. E. Chen, M. Looman, M. Laouri et al., “Burden of illness of diabetic macular edema: literature review,” Current Medical Research and Opinion, vol. 26, no. 7, pp. 1587–1597, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. E. L. Lamoureux and T. Y. Wong, “Diabetic retinopathy in 2011: further insights from new epidemiological studies and clinical trials,” Diabetes Care, vol. 34, no. 4, pp. 1066–1067, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. L. J. Lee, A. P. Yu, K. E. Cahill et al., “Direct and indirect costs among employees with diabetic retinopathy in the United States,” Current Medical Research and Opinion, vol. 24, no. 5, pp. 1549–1559, 2008. View at Publisher · View at Google Scholar · View at Scopus
  8. E. M. Pelletier, B. Shim, R. Ben-Joseph, and J. J. Caro, “Economic outcomes associated with microvascular complications of type 2 diabetes mellitus: results from a US claims data analysis,” Pharmacoeconomics, vol. 27, no. 6, pp. 479–490, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. E. Heintz, A. B. Wirehn, B. B. Peebo, U. Rosenqvist, and L. A. Levin, “Prevalence and healthcare costs of diabetic retinopathy: a population-based register study in Sweden,” Diabetologia, vol. 53, no. 10, pp. 2147–2154, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. Q. Mohamed, M. C. Gillies, and T. Y. Wong, “Management of diabetic retinopathy: a systematic review,” The Journal of the American Medical Association, vol. 298, no. 8, pp. 902–916, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. R. Simó and C. Hernández, “Advances in the medical treatment of diabetic retinopathy,” Diabetes Care, vol. 32, no. 8, pp. 1556–1562, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. M. C. Sugden, P. W. Caton, and M. J. Holness, “PPAR control: it's SIRTainly as easy as PGC,” Journal of Endocrinology, vol. 204, no. 2, pp. 93–104, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. N. D. Lalwani, K. Alvares, and M. K. Reddy, “Peroxisome proliferator-binding protein: identification and partial characterization of nafenopin-, clofibric acid-, and ciprofibrate-binding proteins from rat liver,” Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 15, pp. 5242–5246, 1987. View at Scopus
  14. N. D. Lalwani, W. E. Fahl, and J. K. Reddy, “Detection of a nafenopin binding protein in rat liver cytosol associated with the induction of peroxisome proliferation by hypolipidemic compounds,” Biochemical and Biophysical Research Communications, vol. 116, no. 2, pp. 388–393, 1983. View at Scopus
  15. J. Vamecq and N. Latruffe, “Medical significance of peroxisome proliferator-activated receptors,” The Lancet, vol. 354, no. 9173, pp. 141–148, 1999. View at Publisher · View at Google Scholar · View at Scopus
  16. R. M. Evans, “The steroid and thyroid hormone receptor superfamily,” Science, vol. 240, no. 4854, pp. 889–895, 1988. View at Scopus
  17. L. Fajas, D. Auboeuf, E. Raspé et al., “The organization, promoter analysis, and expression of the human PPARγ gene,” The Journal of Biological Chemistry, vol. 272, no. 30, pp. 18779–18789, 1997. View at Publisher · View at Google Scholar · View at Scopus
  18. O. Braissant, F. Foufelle, C. Scotto, M. Dauça, and W. Wahli, “Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-α, -β, and -γ in the adult rat,” Endocrinology, vol. 137, no. 1, pp. 354–366, 1996. View at Publisher · View at Google Scholar · View at Scopus
  19. D. Auboeuf, J. Rieusset, L. Fajas et al., “Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-α in humans: no alteration in adipose tissue of obese and NIDDM patients,” Diabetes, vol. 46, no. 8, pp. 1319–1327, 1997. View at Scopus
  20. T. Lemberger, O. Braissant, C. Juge-Aubry et al., “PPAR tissue distribution and interactions with other hormone-signaling pathways,” Annals of the New York Academy of Sciences, vol. 804, pp. 231–251, 1996. View at Publisher · View at Google Scholar · View at Scopus
  21. M. del V. Cano and P. L. Gehlbach, “PPAR-α ligands as potential therapeutic agents for wet age-related macular degeneration,” PPAR Research, vol. 2008, Article ID 821592, 5 pages, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. D. Bishop-Bailey, “PPARs and angiogenesis,” Biochemical Society Transactions, vol. 39, pp. 1601–1605, 2011. View at Publisher · View at Google Scholar
  23. J. Zhou, K. M. Wilson, and J. D. Medh, “Genetic analysis of four novel peroxisome proliferator activated receptor-γ splice variants in monkey macrophages,” Biochemical and Biophysical Research Communications, vol. 293, no. 1, pp. 274–283, 2002. View at Publisher · View at Google Scholar · View at Scopus
  24. G. Ding, M. Fu, Q. Qin et al., “Cardiac peroxisome proliferator-activated receptor γ is essential in protecting cardiomyocytes from oxidative damage,” Cardiovascular Research, vol. 76, no. 2, pp. 269–279, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. A. W. Norris, L. Chen, S. J. Fisher et al., “Muscle-specific PPARγ-deficient mice develop increased adiposity and insulin resistance but respond to thiazolidinediones,” The Journal of Clinical Investigation, vol. 112, no. 4, pp. 608–618, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. I. Issemann and S. Green, “Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators,” Nature, vol. 347, no. 6294, pp. 645–650, 1990. View at Publisher · View at Google Scholar · View at Scopus
  27. B. Desvergne and W. Wahli, “Peroxisome proliferator-activated receptors: nuclear control of metabolism,” Endocrine Reviews, vol. 20, no. 5, pp. 649–688, 1999. View at Scopus
  28. D. M. Kendall, C. J. Rubin, P. Mohideen et al., “Improvement of glycemic control, triglycerides, and HDL cholesterol levels with muraglitazar, a dual (α/γ) peroxisome proliferator-activated receptor activator, in patients with type 2 diabetes inadequately controlled with metformin monotherapy: a double-blind, randomized, pioglitazone-comparative study,” Diabetes Care, vol. 29, no. 5, pp. 1016–1023, 2006. View at Publisher · View at Google Scholar · View at Scopus
  29. C. V. Rizos, M. S. Elisaf, D. P. Mikhailidis, and E. N. Liberopoulos, “How safe is the use of thiazolidinediones in clinical practice?” Expert Opinion on Drug Safety, vol. 8, no. 1, pp. 15–32, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. S. E. Nissen, K. Wolski, and E. J. Topol, “Effect of muraglitazar on death and major adverse cardiovascular events in patients with type 2 diabetes mellitus,” The Journal of the American Medical Association, vol. 294, no. 20, pp. 2581–2586, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. R. E. Ratner, S. Parikh, and C. Tou, “Efficacy, safety and tolerability of tesaglitazar when added to the therapeutic regimen of poorly controlled insulin-treated patients with type 2 diabetes,” Diabetes and Vascular Disease Research, vol. 4, no. 3, pp. 214–221, 2007. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Dietz, P. Mohr, B. Kuhn et al., “Comparative molecular profiling of the PPARalpha/gamma activator aleglitazar: PPAR selectivity, activity and interaction with cofactors,” ChemMedChem, vol. 7, no. 6, pp. 1101–1111, 2012. View at Publisher · View at Google Scholar
  33. M. A. Cavender and A. M. Lincoff, “Therapeutic potential of aleglitazar, a new dual PPAR-alpha/gamma agonist: implications for cardiovascular disease in patients with diabetes mellitus,” American Journal of Cardiovascular Drugs, vol. 10, no. 4, pp. 209–216, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. H. A. Pershadsingh and D. M. Moore, “PPARγ agonists: potential as therapeutics for neovascular retinopathies,” PPAR Research, vol. 2008, Article ID 164273, 13 pages, 2008. View at Publisher · View at Google Scholar · View at Scopus
  35. I. Imayama, T. Ichiki, K. Inanaga et al., “Telmisartan downregulates angiotensin II type 1 receptor through activation of peroxisome proliferator-activated receptor γ,” Cardiovascular Research, vol. 72, no. 1, pp. 184–190, 2006. View at Publisher · View at Google Scholar · View at Scopus
  36. D. V. Erbe, K. Gartrell, Y. L. Zhang et al., “Molecular activation of PPARγ by angiotensin II type 1-receptor antagonists,” Vascular Pharmacology, vol. 45, no. 3, pp. 154–162, 2006. View at Publisher · View at Google Scholar · View at Scopus
  37. B. Cariou, Y. Zair, B. Staels, and E. Bruckert, “Effects of the new dual PPAR alpha/delta agonist GFT505 on lipid and glucose homeostasis in abdominally obese patients with combined dyslipidemia or impaired glucose metabolism,” Diabetes Care, vol. 34, no. 9, pp. 2008–2014, 2011. View at Publisher · View at Google Scholar
  38. A. A. Herzlich, X. Ding, D. Shen, R. J. Ross, J. Tuo, and C. C. Chan, “Peroxisome proliferator-activated receptor expression in murine models and humans with age-related macular degeneration,” The Open Biology Journal, vol. 2, pp. 141–148, 2009. View at Publisher · View at Google Scholar
  39. M. A. Dwyer, D. Kazmin, P. Hu, D. P. McDonnell, and G. Malek, “Research resource: nuclear receptor atlas of human retinal pigment epithelial cells: potential relevance to age-related macular degeneration,” Molecular Endocrinology, vol. 25, no. 2, pp. 360–372, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. S. Qin, A. P. McLaughlin, and G. W. De Vries, “Protection of RPE cells from oxidative injury by 15-deoxy-Δ 12,14-prostaglandin J2 by augmenting GSH and activating MAPK,” Investigative Ophthalmology & Visual Science, vol. 47, no. 11, pp. 5098–5105, 2006. View at Publisher · View at Google Scholar · View at Scopus
  41. F. Wang, L. Gao, B. Gong et al., “Tissue-specific expression of PPAR mRNAs in diabetic rats and divergent effects of cilostazol,” Canadian Journal of Physiology and Pharmacology, vol. 86, no. 7, pp. 465–471, 2008. View at Publisher · View at Google Scholar · View at Scopus
  42. A. Tawfik, T. Sanders, K. Kahook, S. Akeel, A. Elmarakby, and M. Al-Shabrawey, “Suppression of retinal peroxisome proliferator-activated receptor γ in experimental diabetes and oxygen-induced retinopathy: role of NADPH oxidase,” Investigative Ophthalmology & Visual Science, vol. 50, no. 2, pp. 878–884, 2009. View at Publisher · View at Google Scholar · View at Scopus
  43. V. Costa, A. Casamassimi, K. Esposito et al., “Characterization of a novel polymorphism in PPARG regulatory region associated with type 2 diabetes and diabetic retinopathy in Italy,” Journal of Biomedicine and Biotechnology, vol. 2009, Article ID 126917, 7 pages, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. M. G. Petrovič, T. Kunej, B. Peterlin, P. Dovč, and D. Petrovič, “Gly482Ser polymorphism of the peroxisome proliferator-activated receptor-γ coactivator-1 gene might be a risk factor for diabetic retinopathy in Slovene population (Caucasians) with type 2 diabetes and the Pro12Ala polymorphism of the PPARγ gene is not,” Diabetes/Metabolism Research and Reviews, vol. 21, no. 5, pp. 470–474, 2005. View at Publisher · View at Google Scholar · View at Scopus
  45. C. Hernandez and R. Simo, “Neuroprotection in diabetic retinopathy,” Current Diabetes Reports, vol. 12, no. 4, pp. 329–337, 2012. View at Publisher · View at Google Scholar
  46. R. Simo and C. Hernandez, “Neurodegeneration is an early event in diabetic retinopathy: therapeutic implications,” British Journal of Ophthalmology, vol. 96, no. 10, pp. 1285–1290, 2012.
  47. E. Carrasco, C. Hernández, A. Miralles, P. Huguet, J. Farrés, and R. Simó, “Lower somatostatin expression is an early event in diabetic retinopathy and is associated with retinal neurodegeneration,” Diabetes Care, vol. 30, no. 11, pp. 2902–2908, 2007. View at Publisher · View at Google Scholar · View at Scopus
  48. M. Garcia-Ramírez, C. Hernández, M. Villarroel et al., “Interphotoreceptor retinoid-binding protein (IRBP) is downregulated at early stages of diabetic retinopathy,” Diabetologia, vol. 52, no. 12, pp. 2633–2641, 2009. View at Publisher · View at Google Scholar · View at Scopus
  49. H. W. van Dijk, F. D. Verbraak, P. H. B. Kok et al., “Decreased retinal ganglion cell layer thickness in patients with type 1 diabetes,” Investigative Ophthalmology & Visual Science, vol. 51, no. 7, pp. 3660–3665, 2010. View at Publisher · View at Google Scholar · View at Scopus
  50. E. Rungger-Brändle, A. A. Dosso, and P. M. Leuenberger, “Glial reactivity, an early feature of diabetic retinopathy,” Investigative Ophthalmology & Visual Science, vol. 41, no. 7, pp. 1971–1980, 2000.
  51. C. Gerhardinger, M. B. Costa, M. C. Coulombe, I. Toth, T. Hoehn, and P. Grosu, “Expression of acute-phase response proteins in retinal Müller cells in diabetes,” Investigative Ophthalmology & Visual Science, vol. 46, no. 1, pp. 349–357, 2005. View at Publisher · View at Google Scholar · View at Scopus
  52. E. Lieth, A. J. Barber, B. Xu et al., “Glial reactivity and impaired glutamate metabolism in short- term experimental diabetic retinopathy. Penn State Retina Research Group,” Diabetes, vol. 47, pp. 815–820, 1998.
  53. E. Lieth, K. F. LaNoue, D. A. Antonetti, and M. Ratz, “Diabetes reduces glutamate oxidation and glutamine synthesis in the retina. The Penn State Retina Research Group,” Experimental Eye Research, vol. 70, no. 6, pp. 723–730, 2000. View at Publisher · View at Google Scholar · View at Scopus
  54. R. A. Kowluru, R. L. Engerman, G. L. Case, and T. S. Kern, “Retinal glutamate in diabetes and effect of antioxidants,” Neurochemistry International, vol. 38, no. 5, pp. 385–390, 2001. View at Publisher · View at Google Scholar · View at Scopus
  55. J. E. Pulido, J. S. Pulido, J. C. Erie et al., “A role for excitatory amino acids in diabetic eye disease,” Experimental Diabesity Research, vol. 2007, Article ID 36150, 7 pages, 2007. View at Publisher · View at Google Scholar · View at Scopus
  56. Y. K. Ng, X. X. Zeng, and E. A. Ling, “Expression of glutamate receptors and calcium-binding proteins in the retina of streptozotocin-induced diabetic rats,” Brain Research, vol. 1018, no. 1, pp. 66–72, 2004. View at Publisher · View at Google Scholar · View at Scopus
  57. A. R. Santiago, J. M. Gaspar, F. I. Baptista et al., “Diabetes changes the levels of ionotropic glutamate receptors in the rat retina,” Molecular Vision, vol. 15, pp. 1620–1630, 2009. View at Scopus
  58. P. Aoun, J. W. Simpkins, and N. Agarwal, “Role of PPAR-γ ligands in neuroprotection against glutamate-induced cytotoxicity in retinal ganglion cells,” Investigative Ophthalmology & Visual Science, vol. 44, no. 7, pp. 2999–3004, 2003. View at Publisher · View at Google Scholar · View at Scopus
  59. A. Bernardo, G. Levi, and L. Minghetti, “Role of the peroxisome proliferator-activated receptor-γ (PPAR-γ) and its natural ligand 15-deoxy-Δ(12,14)-prostaglandin J2 in the regulation of microglial functions,” European Journal of Neuroscience, vol. 12, no. 7, pp. 2215–2223, 2000. View at Publisher · View at Google Scholar · View at Scopus
  60. A. Bernardo, M. A. Ajmone-Cat, L. Gasparini, E. Ongini, and L. Minghetti, “Nuclear receptor peroxisome proliferator-activated receptor-γ is activated in rat microglial cells by the anti-inflammatory drug HCT1026, a derivative of flurbiprofen,” Journal of Neurochemistry, vol. 92, no. 4, pp. 895–903, 2005. View at Publisher · View at Google Scholar · View at Scopus
  61. P. Li, X. Xu, Z. Zheng, B. Zhu, Y. Shi, and K. Liu, “Protective effects of rosiglitazone on retinal neuronal damage in diabetic rats,” Current Eye Research, vol. 36, no. 7, pp. 673–679, 2011. View at Publisher · View at Google Scholar · View at Scopus
  62. E. L. Fletcher, J. A. Phipps, M. M. Ward, K. A. Vessey, and J. L. Wilkinson-Berka, “The renin-angiotensin system in retinal health and disease: its influence on neurons, glia and the vasculature,” Progress in Retinal and Eye Research, vol. 29, no. 4, pp. 284–311, 2010. View at Publisher · View at Google Scholar · View at Scopus
  63. S. Sarlos and J. L. Wilkinson-Berka, “The renin-angiotensin system and the developing retinal vasculature,” Investigative Ophthalmology & Visual Science, vol. 46, no. 3, pp. 1069–1077, 2005. View at Publisher · View at Google Scholar · View at Scopus
  64. N. Nagai, K. Noda, T. Urano et al., “Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor,” Investigative Ophthalmology & Visual Science, vol. 46, no. 3, pp. 1078–1084, 2005. View at Publisher · View at Google Scholar · View at Scopus
  65. M. Schupp, J. Janke, R. Clasen, T. Unger, and U. Kintscher, “Angiotensin type 1 receptor blockers induce peroxisome proliferator-activated receptor-γ activity,” Circulation, vol. 109, no. 17, pp. 2054–2057, 2004. View at Publisher · View at Google Scholar · View at Scopus
  66. R. Simó, M. Villarroel, L. Corraliza, C. Hernández, and M. Garcia-Ramírez, “The retinal pigment epithelium: something more than a constituent of the blood-retinal barrier—implications for the pathogenesis of diabetic retinopathy,” Journal of Biomedicine and Biotechnology, vol. 2010, Article ID 190724, 15 pages, 2010. View at Publisher · View at Google Scholar · View at Scopus
  67. A. V. Ershov and N. G. Bazan, “Photoreceptor phagocytosis selectively activates PPARgamma expression in retinal pigment epithelial cells,” Journal of Neuroscience Research, vol. 60, pp. 328–337, 2000.
  68. N. Toda and M. Nakanishi-Toda, “Nitric oxide: ocular blood flow, glaucoma, and diabetic retinopathy,” Progress in Retinal and Eye Research, vol. 26, no. 3, pp. 205–238, 2007. View at Publisher · View at Google Scholar · View at Scopus
  69. A. Ergul, “Endothelin-1 and diabetic complications: focus on the vasculature,” Pharmacological Research, vol. 63, no. 6, pp. 477–482, 2011. View at Publisher · View at Google Scholar · View at Scopus
  70. J. Kim, Y. S. Oh, and S. H. Shinn, “Troglitazone reverses the inhibition of nitric oxide production by high glucose in cultured bovine retinal pericytes,” Experimental Eye Research, vol. 81, no. 1, pp. 65–70, 2005. View at Publisher · View at Google Scholar · View at Scopus
  71. T. Omae, T. Nagaoka, I. Tanano, and A. Yoshida, “Pioglitazone, a peroxisome proliferator-activated receptor-gamma agonist, induces dilation of isolated porcine retinal arterioles: role of nitric oxide and potassium channels,” Investigative Ophthalmology & Visual Science, vol. 52, no. 9, pp. 6749–6756, 2011. View at Publisher · View at Google Scholar
  72. H. Satoh, K. Tsukamoto, Y. Hashimoto et al., “Thiazolidinediones suppress endothelin-1 secretion from bovine vascular endothelial cells: a new possible role of PPARγ on vascular endothelial function,” Biochemical and Biophysical Research Communications, vol. 254, no. 3, pp. 757–763, 1999. View at Publisher · View at Google Scholar · View at Scopus
  73. D. Ray, M. Mishra, S. Ralph, I. Read, R. Davies, and P. Brenchley, “Association of the VEGF gene with proliferative diabetic retinopathy but not proteinuria in diabetes,” Diabetes, vol. 53, no. 3, pp. 861–864, 2004. View at Publisher · View at Google Scholar · View at Scopus
  74. M. Colucciello, “Vision loss due to macular edema induced by rosiglitazone treatment of diabetes mellitus,” Archives of Ophthalmology, vol. 123, no. 9, pp. 1273–1275, 2005. View at Publisher · View at Google Scholar · View at Scopus
  75. E. H. Ryan Jr., D. P. Han, R. C. Ramsay et al., “Diabetic macular edema associated with glitazone use,” Retina, vol. 26, no. 5, pp. 562–570, 2006. View at Publisher · View at Google Scholar · View at Scopus
  76. N. V. Niemeyer and L. M. Janney, “Thiazolidinedione-induced edema,” Pharmacotherapy, vol. 22, no. 7, pp. 924–929, 2002. View at Scopus
  77. D. S. Fong and R. Contreras, “Glitazone use associated with diabetic macular edema,” American Journal of Ophthalmology, vol. 147, no. 4, pp. 583.e1–586.e1, 2009. View at Publisher · View at Google Scholar · View at Scopus
  78. I. Idris, G. Warren, and R. Donnelly, “Association between thiazolidinedione treatment and risk of macular edema among patients with type 2 diabetes,” Archives of Internal Medicine, vol. 172, no. 13, pp. 1005–1011, 2012. View at Publisher · View at Google Scholar
  79. W. T. Ambrosius, R. P. Danis, D. C. Goff Jr. et al., “Lack of association between thiazolidinediones and macular edema in type 2 diabetes: the ACCORD eye substudy,” Archives of Ophthalmology, vol. 128, no. 3, pp. 312–318, 2010. View at Publisher · View at Google Scholar · View at Scopus
  80. M. Colucciello and E. Ryan, “Macular edema and thiazolidinediones,” Archives of Ophthalmology, vol. 128, no. 12, pp. 1630–1631, 2010. View at Publisher · View at Google Scholar · View at Scopus
  81. K. Muranaka, Y. Yanagi, Y. Tamaki et al., “Effects of peroxisome proliferator-activated receptor γ and its ligand on blood-retinal barrier in a streptozotocin-induced diabetic model,” Investigative Ophthalmology & Visual Science, vol. 47, no. 10, pp. 4547–4552, 2006. View at Publisher · View at Google Scholar · View at Scopus
  82. Z. Zheng, H. Chen, H. Wang et al., “Improvement of retinal vascular injury in diabetic rats by statins is associated with the inhibition of mitochondrial reactive oxygen species pathway mediated by peroxisome proliferator-activated receptor γ coactivator 1α,” Diabetes, vol. 59, no. 9, pp. 2315–2325, 2010. View at Publisher · View at Google Scholar · View at Scopus
  83. D. K. Coletta, A. Sriwijitkamol, E. Wajcberg et al., “Pioglitazone stimulates AMP-activated protein kinase signalling and increases the expression of genes involved in adiponectin signalling, mitochondrial function and fat oxidation in human skeletal muscle in vivo: a randomised trial,” Diabetologia, vol. 52, no. 4, pp. 723–732, 2009. View at Publisher · View at Google Scholar · View at Scopus
  84. K. Yamakawa, M. Hosoi, H. Koyama et al., “Peroxisome proliferator-activated receptor-γ agonists increase vascular endothelial growth factor expression in human vascular smooth muscle cells,” Biochemical and Biophysical Research Communications, vol. 271, no. 3, pp. 571–574, 2000. View at Publisher · View at Google Scholar · View at Scopus
  85. M. Emoto, T. Anno, Y. Sato et al., “Troglitazone treatment increases plasma vascular endothelial growth factor in diabetic patients and its mRNA in 3T3-L1 adipocytes,” Diabetes, vol. 50, no. 5, pp. 1166–1170, 2001. View at Scopus
  86. V. Chintalgattu, G. S. Harris, S. M. Akula, and L. C. Katwa, “PPAR-γ agonists induce the expression of VEGF and its receptors in cultured cardiac myofibroblasts,” Cardiovascular Research, vol. 74, no. 1, pp. 140–150, 2007. View at Publisher · View at Google Scholar · View at Scopus
  87. T. Murata, Y. Hata, T. Ishibashi et al., “Response of experimental retinal neovascularization to thiazolidinediones,” Archives of Ophthalmology, vol. 119, no. 5, pp. 709–717, 2001. View at Scopus
  88. A. Aljada, L. O'Connor, Y. Y. Fu, and S. A. Mousa, “PPARγ ligands, rosiglitazone and pioglitazone, inhibit bFGF- and VEGF-mediated angiogenesis,” Angiogenesis, vol. 11, no. 4, pp. 361–367, 2008. View at Publisher · View at Google Scholar · View at Scopus
  89. A. Higuchi, K. Ohashi, R. Shibata, S. Sono-Romanelli, K. Walsh, and N. Ouchi, “Thiazolidinediones reduce pathological neovascularization in ischemic retina via an adiponectin-dependent mechanism,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 30, no. 1, pp. 46–53, 2010. View at Publisher · View at Google Scholar · View at Scopus
  90. G. A. Rodrigues, F. Maurier-Mahé, D. L. Shurland et al., “Differential effects of PPARγ ligands on oxidative stress-induced death of retinal pigmented epithelial cells,” Investigative Ophthalmology & Visual Science, vol. 52, no. 2, pp. 890–903, 2011. View at Publisher · View at Google Scholar · View at Scopus
  91. H. Hatanaka, N. Koizumi, N. Okumura, et al., “Epithelial-mesenchymal transition-like phenotypic changes of retinal pigment epithelium induced by TGF-β are prevented by PPAR-γ agonists,” Investigative Ophthalmology & Visual Science, vol. 53, no. 11, pp. 6955–6963, 2012. View at Publisher · View at Google Scholar
  92. L. Q. Shen, A. Child, G. M. Weber, J. Folkman, and L. P. Aiello, “Rosiglitazone and delayed onset of proliferative diabetic retinopathy,” Archives of Ophthalmology, vol. 126, no. 6, pp. 793–799, 2008. View at Publisher · View at Google Scholar · View at Scopus
  93. V. Pasceri, H. D. Wu, J. T. Willerson, and E. T. H. Yeh, “Modulation of vascular inflammation in vitro and in vivo by peroxisome proliferator-activated receptor-γ activators,” Circulation, vol. 101, no. 3, pp. 235–238, 2000. View at Scopus
  94. M. Ricote, A. C. Li, T. M. Willson, C. J. Kelly, and C. K. Glass, “The peroxisome proliferator-activated receptor-γ is a negative regulator of macrophage activation,” Nature, vol. 391, no. 6662, pp. 79–82, 1998. View at Publisher · View at Google Scholar · View at Scopus
  95. H. Shu, B. Wong, G. Zhou et al., “Activation of PPARα or γ reduces secretion of matrix metalloproteinase 9 but not interleukin 8 from human monocytic THP-1 cells,” Biochemical and Biophysical Research Communications, vol. 267, no. 1, pp. 345–349, 2000. View at Publisher · View at Google Scholar · View at Scopus
  96. T. Ishizuka, S. Itaya, H. Wada et al., “Differential effect of the antidiabetic thiazolidinediones troglitazone and pioglitazone on human platelet aggregation mechanism,” Diabetes, vol. 47, no. 9, pp. 1494–1500, 1998. View at Scopus
  97. B. P. Harrold, V. J. Marmion, and K. R. Gough, “A double-blind controlled trial of clofibrate in the treatment of diabetic retinopathy,” Diabetes, vol. 18, no. 5, pp. 285–291, 1969. View at Scopus
  98. P. A. Dorne, “Exudative diabetic retinopathy. The use of clofibrate in the treatment of hard exudates using a reduced but prolonged dosage over several years,” Archives d'Ophtalmologie, vol. 37, no. 5, pp. 393–400, 1977. View at Scopus
  99. A. Keech, R. J. Simes, P. Barter et al., “Effects of long-term fenofibrate therapy on cardiovascular events in 9795 people with type 2 diabetes mellitus (the FIELD study): randomised controlled trial,” The Lancet, vol. 366, no. 9500, pp. 1849–1861, 2005. View at Publisher · View at Google Scholar
  100. A. C. Keech, P. Mitchell, P. A. Summanen et al., “Effect of fenofibrate on the need for laser treatment for diabetic retinopathy (FIELD study): a randomised controlled trial,” The Lancet, vol. 370, no. 9600, pp. 1687–1697, 2007. View at Publisher · View at Google Scholar · View at Scopus
  101. E. Y. Chew, W. T. Ambrosius, M. D. Davis et al., “Effects of medical therapies on retinopathy progression in type 2 diabetes,” The New England Journal of Medicine, vol. 363, no. 3, pp. 233–244, 2010. View at Publisher · View at Google Scholar · View at Scopus
  102. R. Simó and C. Hernández, “Fenofibrate for diabetic retinopathy,” The Lancet, vol. 370, no. 9600, pp. 1667–1668, 2007. View at Publisher · View at Google Scholar · View at Scopus
  103. H. N. Ginsberg, M. B. Elam, L. C. Lovato et al., “Effects of combination lipid therapy in type 2 diabetes mellitus,” The New England Journal of Medicine, vol. 362, no. 17, pp. 1563–1574, 2010. View at Publisher · View at Google Scholar · View at Scopus
  104. M. García-Ramírez, F. Canals, C. Hernández et al., “Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy,” Diabetologia, vol. 50, no. 6, pp. 1294–1303, 2007. View at Publisher · View at Google Scholar · View at Scopus
  105. R. Simo, M. Garcia-Ramirez, M. Higuera, and C. Hernandez, “Apolipoprotein A1 is overexpressed in the retina of diabetic patients,” American Journal of Ophthalmology, vol. 147, no. 2, pp. 319.e1–325.e1, 2009. View at Publisher · View at Google Scholar
  106. B. Staels, J. Dallongeville, J. Auwerx, K. Schoonjans, E. Leitersdorf, and J. C. Fruchart, “Mechanism of action of fibrates on lipid and lipoprotein metabolism,” Circulation, vol. 98, no. 19, pp. 2088–2093, 1998. View at Scopus
  107. R. Arakawa, N. Tamehiro, T. Nishimaki-Mogami, K. Ueda, and S. Yokoyama, “Fenofibric acid, an active form of fenofibrate, increases apolipoprotein A-I-mediated high-density lipoprotein biogenesis by enhancing transcription of ATP-binding cassette transporter A1 gene in a liver X receptor-dependent manner,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 25, no. 6, pp. 1193–1197, 2005. View at Publisher · View at Google Scholar · View at Scopus
  108. M. B. Sasongko, T. Y. Wong, T. T. Nguyen, J. E. Shaw, A. J. Jenkins, and J. J. Wang, “Novel versus traditional risk markers for diabetic retinopathy,” Diabetologia, vol. 55, no. 3, pp. 666–670, 2012. View at Publisher · View at Google Scholar
  109. R. Bordet, T. Ouk, O. Petrault et al., “PPAR: a new pharmacological target for neuroprotection in stroke and neurodegenerative diseases,” Biochemical Society Transactions, vol. 34, no. 6, pp. 1341–1346, 2006. View at Publisher · View at Google Scholar · View at Scopus
  110. H. Murakami, R. Murakami, F. Kambe et al., “Fenofibrate activates AMPK and increases eNOS phosphorylation in HUVEC,” Biochemical and Biophysical Research Communications, vol. 341, no. 4, pp. 973–978, 2006. View at Publisher · View at Google Scholar · View at Scopus
  111. M. Zanetti, A. Stocca, B. Dapas et al., “Inhibitory effects of fenofibrate on apoptosis and cell proliferation in human endothelial cells in high glucose,” Journal of Molecular Medicine, vol. 86, no. 2, pp. 185–195, 2008. View at Publisher · View at Google Scholar · View at Scopus
  112. A. Tomizawa, Y. Hattori, T. Inoue, S. Hattori, and K. Kasai, “Fenofibrate suppresses microvascular inflammation and apoptosis through adenosine monophosphate-activated protein kinase activation,” Metabolism, vol. 60, no. 4, pp. 513–522, 2011. View at Publisher · View at Google Scholar · View at Scopus
  113. J. Kim, J. H. Ahn, J. H. Kim et al., “Fenofibrate regulates retinal endothelial cell survival through the AMPK signal transduction pathway,” Experimental Eye Research, vol. 84, no. 5, pp. 886–893, 2007. View at Publisher · View at Google Scholar · View at Scopus
  114. S. Miranda, A. Gonzalez-Rodriguez, M. Garcia-Ramirez et al., “Beneficial effects of fenofibrate in retinal pigment epithelium by the modulation of stress and survival signaling under diabetic conditions,” Journal of Cellular Physiology, vol. 227, no. 6, pp. 2352–2362, 2012.
  115. D. A. Antonetti, R. Klein, and T. W. Gardner, “Diabetic retinopathy,” The New England Journal of Medicine, vol. 366, pp. 1227–1239, 2012. View at Publisher · View at Google Scholar
  116. G. Chinetti, S. Griglio, M. Antonucci et al., “Activation of proliferator-activated receptors α and γ induces apoptosis of human monocyte-derived macrophages,” The Journal of Biological Chemistry, vol. 273, no. 40, pp. 25573–25580, 1998. View at Publisher · View at Google Scholar · View at Scopus
  117. Z. Israelian-Konaraki and P. D. Reaven, “Peroxisome proliferator-activated receptor-alpha and atherosclerosis: from basic mechanisms to clinical implications,” Cardiology, vol. 103, no. 1, pp. 1–9, 2005. View at Publisher · View at Google Scholar · View at Scopus
  118. M. Villarroel, M. Garcia-Ramirez, L. Corraliza, C. Hernandez, and R. Simo, “Fenofibric acid prevents retinal pigment epithelium disruption induced by interleukin-1beta by suppressing AMP-activated protein kinase (AMPK) activation,” Diabetologia, vol. 54, no. 6, pp. 1543–1553, 2011. View at Publisher · View at Google Scholar
  119. K. Trudeau, S. Roy, W. Guo et al., “Fenofibric acid reduces fibronectin and collagen type IV overexpression in human retinal pigment epithelial cells grown in conditions mimicking the diabetic milieu: functional implications in retinal permeability,” Investigative Ophthalmology & Visual Science, vol. 52, pp. 6348–6354, 2011. View at Publisher · View at Google Scholar
  120. I. Inoue, K. Shino, S. Noji, T. Awata, and S. Katayama, “Expression of peroxisome proliferator-activated receptor α (PPARα) in primary cultures of human vascular endothelial cells,” Biochemical and Biophysical Research Communications, vol. 246, no. 2, pp. 370–374, 1998. View at Publisher · View at Google Scholar · View at Scopus
  121. M. Meissner, M. Stein, C. Urbich et al., “PPARα activators inhibit vascular endothelial growth factor receptor-2 expression by repressing Sp1-dependent DNA binding and transactivation,” Circulation Research, vol. 94, no. 3, pp. 324–332, 2004. View at Publisher · View at Google Scholar · View at Scopus
  122. J. Varet, L. Vincent, P. Mirshahi et al., “Fenofibrate inhibits angiogenesis in vitro and in vivo,” Cellular and Molecular Life Sciences, vol. 60, no. 4, pp. 810–819, 2003. View at Publisher · View at Google Scholar · View at Scopus
  123. Y. Yokoyama, B. Xin, T. Shigeto et al., “Clofibric acid, a peroxisome proliferator-activated receptor α ligand, inhibits growth of human ovarian cancer,” Molecular Cancer Therapeutics, vol. 6, no. 4, pp. 1379–1386, 2007. View at Publisher · View at Google Scholar · View at Scopus
  124. Y. Chen, Y. Hu, M. Lin et al., “Therapeutic effects of PPARalpha agonists on diabetic retinopathy in type 1 diabetes models,” Diabetes, vol. 62, no. 1, pp. 261–272, 2013. View at Publisher · View at Google Scholar
  125. R. L. Stephen, M. C. U. Gustafsson, M. Jarvis et al., “Activation of peroxisome proliferator-activated receptor delta stimulates the proliferation of human breast and prostate cancer cell lines,” Cancer Research, vol. 64, no. 9, pp. 3162–3170, 2004. View at Publisher · View at Google Scholar · View at Scopus