15-deoxy-Δ12,14-prostaglandin J2 Down-Regulates Activin-Induced Activin Receptor, Smad, and Cytokines Expression via Suppression of NF-κB and MAPK Signaling in HepG2 Cells
Figure 2
Activin receptor and Smad mRNA expression in HepG2 cells. HepG2 cells were treated with activin A (25 ng/mL) and 15d-PGJ2 (2 or 5 μM) for 72 h, and levels of ActR (a) and Smad (b) mRNA were measured by real-time PCR. The crossing point of activin receptors with β-actin was entered into the formula, 2−(target gene-β actin), and the relative amounts were quantified. The data represent the mean ± SEM of 3 independent samples. *, comparison of stimulation with 15d-PGJ2 and activin A versus activin A stimulation alone.
