Effects of Three Different Fibrates on Intrahepatic Cholestasis Experimentally Induced in Rats

Table 2

Effect of pretreatment of EE and CPZ treated rats with PPAR𝛼 receptor antagonist (GW6471) 1 mg/kg/d i.p 30 min before fenofibrate, bezafibrate, or gemfibrozil on biochemical parameters and bile flow rate.

Parameter	GW6471	EE + CPZ	Feno + GW	Beza + GW	Gem + GW
ALP U/L	𝟐𝟐𝟔.𝟗±22.9^a	𝟓𝟓𝟔.𝟓𝟗±69.5^∗b	𝟓𝟏𝟒.𝟗𝟖±57.4^∗b∧	𝟒𝟗𝟑.𝟔𝟔±35.4^∗ab∧	𝟒𝟏𝟏.𝟖𝟐±22,6^∗ab∧
GGT U/L	𝟖.𝟔𝟗±1.3^a	𝟏𝟗.𝟗±5.4^∗b	𝟏𝟔.𝟓𝟔±4.85^∗b∧	𝟗.𝟎𝟏±2.25^∗ab∧	𝟏𝟓.𝟗𝟕±5.23^∗b∧
AST U/mL	𝟔𝟕.𝟗𝟑±9.6^a	𝟏𝟓𝟎.𝟔𝟗±15.6^∗b	𝟏𝟒𝟖.𝟏𝟖±20.96^∗b∧	𝟗𝟖.𝟎𝟑±11.19^∗a∧bF	𝟏𝟏𝟏.𝟕𝟗±19.04^∗ab∧F
ALT U/mL	𝟒𝟗.𝟏𝟑𝟕±7.99^a	𝟖𝟓.𝟓±11.83^∗b	𝟖𝟔.𝟏𝟑𝟐±11.97^∗b∧	𝟕𝟕.𝟐𝟏±13.36^∗ab∧	𝟕𝟑.𝟑𝟑±7.398^∗ab∧F
TBA 𝜇mole/L	𝟏𝟔.𝟏𝟏𝟔±4.104^a	𝟖𝟏.𝟕𝟓±20.2^∗b	𝟕𝟗.𝟔𝟓±15.92^∗b∧	𝟕𝟐.𝟖𝟓±19.35^∗𝑎∧bF	𝟔𝟏.𝟔𝟗±14.9^∗ab∧F
Direct Bil. 𝜇mole/L	𝟎.𝟗𝟎𝟐𝟓±0.316^a	𝟐.𝟎𝟔𝟓±0.36^∗b	𝟏.𝟗𝟕±0.418^∗b∧	𝟏.𝟑𝟖𝟕±0.28^∗𝑎∧bF	𝟏.𝟐𝟖𝟔±0.329^∗ab∧F
T. BIL. μmole/L	𝟏.𝟗𝟕±0.722^a	𝟓.𝟒𝟐±1.39^∗b	𝟓.𝟔𝟑𝟕±1.83^∗b∧	𝟑.𝟕𝟖𝟔±1.318^∗𝑎∧bF	𝟑.𝟑𝟓𝟑±0.79^∗ab∧F
IL-1𝛽 pg/mL	𝟎.𝟏𝟎𝟗±0.013^a	𝟎.𝟐𝟕±0.059^∗b	𝟎.𝟐𝟒𝟑𝟓±0.099^∗b∧	𝟎.𝟏𝟔𝟗𝟒±0.043^∗ab∧	𝟎.𝟏𝟓𝟒𝟏±0.037^∗ab∧F
TNF𝛼 ng/mL	𝟎.𝟏𝟎𝟏±0.09^a	𝟎.𝟏𝟔𝟒𝟖±𝟎.𝟐𝟗^∗b	𝟎.𝟏𝟓𝟓±0.2^∗b∧	𝟎.𝟎𝟗𝟑𝟗±0.05^∗ab∧F	𝟎.𝟏𝟐𝟑𝟐±0.01^∗a∧bF
MDA 𝜇mole/gm tissue	𝟐𝟗.𝟏𝟓±11.53^a	𝟕𝟓.𝟗𝟔±12.84^∗b	𝟔𝟗.𝟑𝟖±21.3^∗b∧	𝟓𝟗.𝟔𝟕±19.11^∗ba∧	𝟒𝟔.𝟏𝟐±15.9^∗ab∧F
Bile flow rate 𝜇L/min/Kg b·wt	𝟏𝟗.𝟖𝟕±1.09^a	𝟑.𝟖𝟔𝟕𝟓±2.3^∗b	𝟓.𝟏𝟑±1.33^∗b∧	𝟕.𝟗𝟖±1.34^∗ab∧F	𝟏𝟎.𝟎𝟓𝟓±1.02^∗ab∧F

ALP: alkaline phosphatase; GGT: gamma glutamyl transpeptidase; AST: aspartate aminotransferase; ALT: alanine aminotransferase; TBA: total bile acids; Direct Bil: direct bilirubin; T. Bil.: total bilirubin; IL-1𝛽: interleukin-1beta; TNF𝛼: tumor necrosis factor alpha; MDA: malondialdehyde; EE: ethinylestradiol; CPZ: chlorpromazine; Feno: fenofibrate; Beza: bezafibrate; Gem: gemfibrozil; GW: GW6471. Data are presented as mean ± SD. ∗Significantly different from control, asignificantly different from EE and CPZ groups, bsignificantly different from GW6471 group, ∧significantly different from corresponding group not receiving GW6471, Fsignificantly different from (feno + GW6471) group at 𝑃<0.05.