Figure 2: Gefitinib dose response in human HK2 cells showing a complete inhibition of high-glucose- (HG-) induced P-EGFR over t-EGFR normalized to Actin (a) and P-ErK1/2 over t-ErK1/2 normalized to Actin (b) in the presence of 0.1–1 μM of gefitinib. HK2 cells were treated for 48 hrs. Western blot was determined as described in Section 2. Representative images are shown. Normalized results are expressed as mean ± SEM; . * and # versus control.