Figure 3: High glucose and pioglitazone induced AQP1 are mediated through EGFR phosphorylation. HK2 cells were incubated for 48 h with 5 mM glucose media (control), high glucose (HG) ± Piog (10 μM) ± gefitinib (0.5 μM) and levels of AQP1 were determined by Western blotting. Representative images for AQP1 and actin bands are shown. Normalized results are expressed as mean ± SEM; . # versus control and δ versus HG.