Combined Effects of PPARγ Agonists and Epidermal Growth Factor Receptor Inhibitors in Human Proximal Tubule Cells

Figure 5

High-glucose-induced fibronectin expression. Pioglitazone and gefitinib reduced high-glucose-induced fibronectin expression. Combination of both pioglitazone and gefitinib has a similar effect. HK2 cells were incubated for 48 h with 5 mM glucose media (control), high glucose (HG) ± Piog (10 μM) ± gefitinib (0.5  μM) and levels of fibronectin were determined by Western blotting. Representative images for fibronectin and actin bands are shown. Normalized results are expressed as mean ± SEM; 𝑛=4. ^δ𝑃<0.005 versus control and *𝑃<0.05 versus HG and ^‡𝑃<0.05 versus HG+Piog.