982462.fig.007
Figure 7: High-glucose-induced DNA binding of DIG-labelled AP-1. AP-1-DNA binding activity was significantly increased with high glucose. Pioglitazone reduces high-glucose-induced AP-1 binding activity. Gefitinib completely abolished AP-1 binding activity induced by HG. HK2 cells were incubated for 72 hrs with 5 mM glucose media (Control), high glucose (HG) ± Piog (10μM) ± gefitinib (0.5μM). Nuclear extract preparation and EMSA are as described in Section 2. A representative image showing the shift band of AP-1 is shown. Quantitative data are expressed as mean ± SEM; . δ versus control; * versus Control and versus H iog.