Combined Effects of PPARγ Agonists and Epidermal Growth Factor Receptor Inhibitors in Human Proximal Tubule Cells

Figure 7

High-glucose-induced DNA binding of DIG-labelled AP-1. AP-1-DNA binding activity was significantly increased with high glucose. Pioglitazone reduces high-glucose-induced AP-1 binding activity. Gefitinib completely abolished AP-1 binding activity induced by HG. HK2 cells were incubated for 72 hrs with 5 mM glucose media (Control), high glucose (HG) ± Piog (10  μM) ± gefitinib (0.5  μM). Nuclear extract preparation and EMSA are as described in Section 2. A representative image showing the shift band of AP-1 is shown. Quantitative data are expressed as mean ± SEM; 𝑛=3. ^δ𝑃<0.005 versus control; *𝑃<0.05 versus Control and ^‡𝑃<0.001 versus HG+Piog.