Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry
Figure 1
Purification of PPARγ proteins from 293T and Caco-2 cell lysates. (a) Schematic diagram of the purification of the exogenous and endogenous PPARγ proteins. 293T and Caco-2 cell lysates were incubated with anti-PPARγ cross-linked Protein G Dynabeads as described in Section 2. Rabbit IgG cross-linked Protein G Dynabeads were used as a negative control. Bound proteins were eluted with 0.1 M glycine-HCl buffer (pH 2). (b) Isolated exogenous and endogenous PPARγ proteins. Eluted proteins were subjected to SDS-PAGE, followed by silver staining. The molecular masses of PPARγ1 and PPARγ2 are shown in the right side of the gel. (c) Enrichment of PPARγ proteins were also confirmed by Western blotting using anti-PPARγ specific antibodies.
