Identification of Posttranslational Modifications in Peroxisome Proliferator-Activated Receptor γ Using Mass Spectrometry
Figure 3
Identified PTMs in PPARγ2 proteins from 293T cells. (a) Representative MS/MS spectra of PPARγ2 proteins of the peptide [99-PPYYSEK-119] assigned by MODIRO are shown. (b) List of identified PTMs in exogenous PPARγ2 proteins using ion trap MS/MS. Amino acid sequences, position of amino acids, identified PTMs theoretical mass, measured mass, error between measured and theoretical masses in Daltons, and significance score are listed. (c) Diagram for summary of identified PTMs in exogenous PPARγ2 proteins. (d) Identified PTMs in exogenous PPARγ2 proteins using FT MS/MS. Each responsible spectrum for S112 (top) and T487 (bottom) is shown.
