PPARG in Human Adipogenesis: Differential Contribution of Canonical Transcripts and Dominant Negative Isoforms
Figure 4
Transcript-specific RT-PCR assays for PPARG canonical transcripts (panel (a)) and ORF4 variants (panel (b)) at different time points of the adipogenesis (indicated on the top). On the left, the different PPARG transcripts are schematically shown; on the right the related PCR amplicons and their sizes (in bp) are illustrated. “PPARGt” and “ORF4t” indicate the entire pool of canonical PPARG and ORF4 transcripts, respectively. Transcript-specific exons are shown in grey and common exons are coloured.Black arrows indicate the specific primer pairs used in this analysis (Fw, forward; Rv, reverse). GAPDH was used as internal control.
