PGC1α −1 Nucleosome Position and Splice Variant Expression and Cardiovascular Disease Risk in Overweight and Obese Individuals

Figure 1

PGC1 −1 nucleosome position. (a) Scanning qPCR was performed using four overlapping primer pairs targeted to the PGC1 promoter region from approximately the −800 nt to the −100 nt and depicted in the schematic. (b) Nucleosome positions were determined based on densitometry results and plotted as a line graph for each individual. All participants showed similar amplification with primer pairs 1 and 2, depicted as an upstream nucleosome (gray). Similar amplification was also seen with primer pair 3 but not with primer pair 4. This amplification pattern is depicted as a phased −1N positioned between −170 nt and −440 nt (white and black) below the line graph. Based on the phased −1N position, participants were divided into two experimental groups shown on the right: upstream (UP, black) and downstream (DN, white), and densitometry results for each group are shown as mean ± SEM in the bar graphs. −1N for UP and DN is depicted beneath each bar graph. All nucleosome positions are depicted relative to the transcriptional start site (TSS).

(a) Scanning PCR primers

(b) PGC1   −1 nucleosome position and subject division