PPAR Research

Research Article

Treatment with PPARα Agonist Clofibrate Inhibits the Transcription and Activation of SREBPs and Reduces Triglyceride and Cholesterol Levels in Liver of Broiler Chickens

Table 2

Characteristics and performance data of the primers used for reference gene-stability measure and quantitative real-time PCR analysis.
Gene namePrimer sequence (forward, reverse)Product size (bp)NCBI GenBank
Reference genes   
 ATP5BGGTGTGCAGAAGATCCTTCA, GATCTGCTTGAAGCCCTTGA216NM_001031391
 TOP1GCATCATGCCAGAAGACATC, CTTGAGCTAGGGTTCAACATT175NM_205110
 MDH1GCATGGAGAGGAAGGATTTG, GAAGTCACACCAAGCTTCAG241NM_001006395
 RPL13GTTCGTGCTGGCAGAGGAT, GACAGCTGAGTTGCCATCTT245NM_204999
 YWHAZTGTGGAGCAATCACAACAGG, GTTGTCTCTCAGTAACTGCAT250NM_001031343
 GAPDHCACTTCAAGGGCACTGTCAA, CTCATGGTTGACACCCATCA252NM_204305
Target genes   
 PPARαGGAGTTTAAGTGACCGCTCT, CTGATCCATCAGATCCTGGA230NM_001001464
 CPT1AACTCTCCAGCACGTGAAAGA, CCTGCAGTAAGAGCTGCTAA118NM_001012898
 SREBF1ACCTGGCAGCCAAGGCAT, GACTCAGCCATGATGCTTCT168NM_204126
 FASNCTCCTTGAAGGTGGTTTGCA, CCTCCATGTTTCCTGCTTTC219NM_205155
 GPAMGTTGAGACAGCAGCAGTTTTT, CCTTCAATTATGCGATCGTAG177XM_421757
 SREBF2CATTCTGACCACAATGCCAG, GGTCCTTCAGCTCAATGATC176AJ414379
 HMGCRGCTTAGCCTTTCTCCTTGCT, CCAGATTGTTTCCTGCAGCA250XM_422225
 LDLRCACTCAGTGCCACCATTTGG, CTGCGACGGAACGTCCAAG203NM_204452
 INSIG1CCAACAATGTCCAGCTGTCC, CACCATTATATACAAGGAACTG147NM_001030966
 INSIG2GGATTTTGCCAACAATATCCAG,TGTAGACCAGAAGCTGTGACA,149NM_001031261
 LXRαCCAAGATGCTGGGAAATGAAGC, ATATACATGTCCATCTCACAC193NM_204542
Sterol regulatory element binding transcription factors 1 and 2 (SREBF1 and SREBF2); peroxisome proliferator-activated receptor alpha (PPARα); carnitine palmitoyltransferase 1A (CTP1A); fatty acid synthase (FASN); glycerol-3-phosphate acyltransferase, mitochondrial (GPAM); 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR), low-density lipoprotein receptor (LDLR); Liver X-Receptor alpha (LXRα); insulin induced genes 1 and 2 (INSIG1 and INSIG2); ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B); topoisomerase (DNA) I (TOP1); malate dehydrogenase 1, NAD (soluble) (MDH1); ribosomal protein L13 (RPL13); tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ); glyceraldehyde-3-phosphate dehydrogenase (GAPDH).