Smad2/3 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells

Figure 3

Relationship between PPARα and Smad2/3 in DBP-treated SCs. GW6471, WY14643, and SB431542 were added to the cultural medium 2 h before addition of DBP, respectively. Western blotting was used to test levels of p-Smad2/3 and Smad2/3 in WY14643 + DBP- and GW6471 + DBP-treated SCs (as shown in (a)) and levels of PPARα in DBP- and SB431542 + DBP-treated SCs, respectively (as shown in (c)). (b) and (d) show the relative quantitative analysis of parts (a) and (c), respectively. was considered statistically significant.

(a)

(b)

(c)

(d)