15-Deoxy-Δ^12,14-Prostaglandin J₂ Inhibits Homing of Bone Marrow-Derived Mesenchymal Stem Cells Triggered by Chronic Liver Injury via Redox Pathway

Figure 1

15d-PGJ₂ inhibits the migration of BMSCs toward injured liver. ((a) and (b)) 4 weeks of CCl₄ were used to induce mouse liver fibrosis with or without 15d-PGJ₂ administration ( per group). Total MSCs were isolated from the NPCs in the liver by flow cytometry analysis based on CD166 and CD105. Representative histograms and proportions of CD166⁺ (a) and CD105⁺ (b) in NPCs. ((c)–(f)) Mice were lethally irradiated and received whole BM transplants from EGFP transgenic mice, followed by CCl₄ injection for 4 weeks in the presence or absence of 15d-PGJ₂ treatment. BMSCs in the liver NPCs were identified as CD166⁺/EGFP⁺ or CD105⁺/EGFP⁺. ((c) and (e)) Representative histograms of BMSCs. ((d) and (f)) The proportions of CD166⁺/EGFP⁺ BMSCs and CD166⁺/EGFP⁻ resident MSCs (d) and CD105⁺/EGFP⁺ BMSCs and CD105⁺/EGFP⁻ resident MSCs in NPCs of liver tissues. , compared with olive oil (OO) group. , compared with CCl₄ group without 15d-PGJ₂ ().

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