Research Article
15-Deoxy-Δ12,14-Prostaglandin J2 Inhibits Homing of Bone Marrow-Derived Mesenchymal Stem Cells Triggered by Chronic Liver Injury via Redox Pathway
Figure 2
Effects of 15d-PGJ2 on BMSCs migration and proliferation. ((a) and (b)) Flow cytometry analysis was performed to identify BMSCs based on the specific marker of CD166 (a) and CD105 (b). (c) BMSCs were exposed to varying concentrations of 15d-PGJ2 for 24 hours. Cell Counting Kit-8 was used to assess cell proliferation. ((d)–(f)) Serum-starved BMSCs were preincubated with indicated doses of 15d-PGJ2 for 12 hours and then allowed to migrate for 4 hours with or without PDGF (30 ng/mL) administration. Representative images of migration of BMSCs (d). Quantitative analysis of BMSC migration in the presence (f) or absence (e) of PDGF. , compared with control (). Scale bars, 50 μm.
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