15-Deoxy-Δ^12,14-Prostaglandin J₂ Inhibits Homing of Bone Marrow-Derived Mesenchymal Stem Cells Triggered by Chronic Liver Injury via Redox Pathway

Figure 3

15d-PGJ₂ inhibits BMSCs migration independently of PPARγ. (a) Serum-starved BMSCs were pretreated with or without 10 μM GW9662 for 1 hour and then exposed to 5 μM 15d-PGJ₂ or 10 μM troglitazone (Trog) or ciglitazone (Cig) for 12 hours and were subsequently allowed to migrate for 4 hours. ((b)–(d)) Serum-starved BMSCs were incubated with 15d-PGJ₂ (5 μM) for 12 hours. Real-time RT-PCR was performed to determine mRNA expression of PPARγ in BMSCs (b). PPARγ protein expression was detected by immunofluorescence analysis (green) (d), and total fluorescence intensity of PPARγ was counted with high content analysis (c). , compared with control (). Scale bars, 50 μm.

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