PPARα Is Required for PPARδ Action in Regulation of Body Weight and Hepatic Steatosis in Mice

Figure 1

Treatment with GW501516 prevents diet induced weight gain and changes the hepatic TG level in non-tg animals. (a) GW501516 decreased body mass accumulation, expressed as percentage of initial body weight. (b) Following 4 weeks of treatment, TG in the liver increased by 91% () when compared to control animals and after 8 weeks of treatment, hepatic TG levels had returned (decreased by 73%) to levels observed at the 2-week time point in both groups (). (c) Liver section stained for fat with Oil Red O. Level of red colour indicates presence of lipid droplets. (d) Chosen hepatic gene expression shows no change in mRNA levels of any member of PPAR family throughout the experiment. Lipogenic gene Fas expression was downregulated () after 4 weeks, while β-oxidation markers Cpt1 and Acox1 were upregulated () or remained without change, respectively (4 weeks). mice/group, two-way ANOVA was used to calculate significance.

(a)

(b)

(c)

(d)