A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue

Figure 2

Effect of 10 and 100 μg/mL Citrus bergamia extract (CBE) on the lipid content of mesenchymal stem cells stained with oil red O during adipogenic differentiation. Cells were examined at 7, 14, 21, and 28 days from the start of differentiation. (a) A representative photograph from three independent experiments of untreated control (Control), adipogenic medium-treated cells (AM), and AM plus 10 or 100 μg/mL CBE was shown. (b) The levels of oil red O incorporation were quantified by measuring the absorbance of isopropyl alcohol extract at  nm. Data were shown relative to AM and expressed as mean ± SD from three independent experiments. versus AM (100%) at same day; versus CBE 10 μg/mL at 7 days; versus CBE 100 μg/mL at 7 days.

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