The Correlation of PPARα Activity and Cardiomyocyte Metabolism and Structure in Idiopathic Dilated Cardiomyopathy during Heart Failure Progression

Table 2

Primers used for quantitative real-time polymerase chain reaction.

Gene	Primer sequence
PPARα	F: 5′-AGA-TTT-CGC-AAT-CCA-TCG-GC-3′
	R: 5′-GCG-TGG-ACT-CCG-TAA-TGA-TA-3′
FAT/CD36	F: 5′-GGA-AAG-TCA-CTG-CGA-CAT-GA-3′
	R: 5′-CCT-TGG-ATG-GAA-GAA-CGA-ATC-3′
CPT-1	F: 5′-GGT-GAA-CAG-CAA-CTA-TTA-TGT-C-3′
	R: 5′-ATC-CTC-TGG-AAG-TGC-ATC-3′
GLUT4	F: 5′-GCT-ACC-TCT-ACA-TCA-TCC-AGA-ATC-TC-3′
	R: 5′-CCA-GAA-ACA-TCG-GCC-CA-3′
PDK-4	F: 5′-TAC-TCC-ACT-GCA-CCA-ACG-C-3′
	R: 5′-AAT-TGG-CAA-GCC-GTA-ACC-A-3′
Beclin-1	F: 5′-TGG-ATC-ACC-CAC-TCT-GTG-AG-3′
	R: 5′-TTA-TTG-GCC-AGA-GCA-TGG-AG-3′
Caspase-3	F: 5′-AGA-ACT-GGA-CTG-TGG-CAT-TGA-G-3′
	R: 5′-GCA-TTG-TCG-GCA-TAC-TGT-TTC-AG-3′
GAPDH	F: 5′-GAA-GTA-GGT-GAT-GGG-ATT-TC-3′
	R: 5′-CAA-GCT-TCC-CGT-TCT-CAG-CC-3′
β-actin	F: 5′-TGC-CAT-CCT-AAA-AGC-CAC-3′
	R: 5′-TCA-ACT-GGT-CTC-AAG-TCA-GTG-3′