Differential Roles of Peroxisome Proliferator-Activated Receptor-α and Receptor-γ on Renal Crystal Formation in Hyperoxaluric Rodents

Figure 1

Renal CaOx crystal formation and PPAR expression in hyperoxaluric mice. (a) Quantitative estimation of renal CaOx crystals. Crystallization in each kidney section on designed time points was quantified by calculating the ratio (%) of the area containing crystals to that of the entire kidney section by using Image Pro Plus. (b) Evaluation of PPARα and PPARγ expression by qRT-PCR using TaqMan assays ( for each time point). Control values represent the average on day 0 and data are presented as mean ± SE. versus gene expression on day 0. (c) PPARα and PPARγ immunohistochemistry in hyperoxaluric mouse kidneys obtained on days 0, 6, and 12. Each asterisk represents the location of CaOx crystals detected by Pizzolato staining. Magnification: 400x. Scale bar = 50 μm.

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