Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark®) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens
Figure 1
Configuration of the H2T and H2D assay. (a) Configuration of the HER2 total assay consists of two antibodies specific to unique epitopes on the c-terminus of the HER2 protein. One antibody (Ab-1) is conjugated to a VeraTag molecule (V) and the other is conjugated to biotin (Ab-2). The photosensitizer molecule (PM) brings the strepavidin-methylene blue in close proximity to the antibodies. (b) Configuration of the H2D assay consists of one antibody that is specific for a unique epitope on the HER2 protein. The same antibody is conjugated either to a VeraTag (V; Ab-1) or to biotin (Ab-1). The photosensitizer molecule (PM) brings the strepavidin-methylene blue in close proximity to the antibodies.
