Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark®) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens
Figure 3
Accuracy of the H2T and H2D assays. Seven ((a); H2T) and six ((b); H2D) different cell lines with varying levels of HER2 total protein, as measured by in house ELISA and flow cytometry, were run in the H2T assay in two separate batches. All signals showed correct rank order and accuracy based on ELISA and flow cytometry comparisons.
