Morphological Analysis of CDC2 and Glycogen Synthase Kinase 3β Phosphorylation as Markers of G2 → M Transition in Glioma

Figure 1

CDC2 pathway is regulated principally by post-translational modification. CDC2 phosphorylation at Tyr15 by Wee1/Myt leads to inactivation of CDC2 and is reversed by the dephosphorylation activity of CDC25A. CDC2 activation is mediated through phosphorylation by cyclin activating kinase (CAK/CDK7) at Threonine 161. Once phosphorylated, CDC2 may promote mitosis by interaction with Cyclins A and B. GSK3β is a target of EGFR and may inhibit CDC25’s phosphorylation activity, indirectly leading to the inactivation of CDC2. Pathway is adapted from KEGG pathways hsa04110 and has 04115.