Morphological Analysis of CDC2 and Glycogen Synthase Kinase 3β Phosphorylation as Markers of G2 → M Transition in Glioma
Figure 5
pSer9GSK3β labeling in glial tumors. Tissue arrays for GBM (a–c), oligodendroglioma (d–f), and ependymoma (g–h) were stained with pSer9GSK3β antibodies. Groups were separated into low labeling (a, d, and g), intermediate labeling (b, e, and h), and strong labeling intensity (c and f) as described in Section 2, and pictures representative of these groups for the three tumors is presented. The number of cores positive and their corresponding percentages are placed in the bottom left of each panel. Perinuclear/cytoplasmic labeling predominates (arrow, (f)), but nuclear (arrowhead, (c)) and labeling in glial processes also was found (double arrows, (c)). (GBM = glioblastoma; Oligo = oligodendroglioma; EP = ependymoma; scale bar in yellow represents 100 μm).
