Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast
Figure 2
Serial archival sections representative of an IDC stained for H&E, TOP2A, MCM2 and BUB1B proteins. (a) Primary invasive ductal carcinoma (IDC) of the breast, grade 2, featuring focal tubular differentiation. (b, c, d) Distinct nuclear immunoreactivity for TOP2A marking the presence of cycling cells in about 15% of the infiltrating tumor cells, and for MCM2 marking the “licensed” population in about 1/3rd of the infiltrating tumor cells and diffuse cytoplasmic immunoreactivity (2+) with focal cell membrane accentuation for BUB1B protein (Immunoperoxidase staining (IMPOX staining); original magnifications 200x).
