Novel Molecular Markers of Malignancy in Histologically Normal and Benign Breast

Table 4

TOP2A, MCM2, and BUB1B protein expression scores in IDCs, molecularly low-risk and molecularly high risk, histologically normal breast tissues.

Case no.	Breast tissue specimen category (based on gene expression profiling)	Histologic tumor type on initial biopsy/lumpectomy	Final pathologic diagnosis on mastectomy	Histopathologic findings on archival tissue sections selected for IHC validation	Topoisomerase II-alpha (TOP2A) index (%)	MCM2-index (%)	BUB1B protein expression score (H-score)
1	Carcinoma	IDC	IDC, DCIS	Invasive Cancer	25	30	170
2	Carcinoma	IDC	Invasive papillary CA with a focus of IDC	Invasive Cancer	30	30	135
3	Carcinoma	IDC	IDC	Invasive cancer	15	80	145
4	Carcinoma	IDC	IDC	Invasive Cancer	35	60	80
5	Carcinoma	IDC	IDC	Invasive Cancer	25	50	165
6	Carcinoma	IDC, ILC	IDC w/ lobular features	Invasive cancer	30	30	200
HNB tissues with low-grade molecular abnormality (HNB-LGMA)
7	HNB-LGMA 1	IDC	IDC	Benign breast tissue	2.5	2	21
8	HNB-LGMA 2	IDC	IDC	Benign breast tissue	2.5	6	20
9	HNB-LGMA 3	IDC	IDC	Benign breast tissue	2.5	12	22
10	HNB-LGMA 4	Mucinous carcinoma	No residual mucinous carcinoma	Benign breast tissue	1	2	15
11	HNB-LGMA 5	DCIS	Residual DCIS, multifocal	Benign breast tissue	1	1	10
12	HNB-LGMA 6	IDC	No residual IDC	Benign breast tissue	1	1	18
13	HNB-LGMA 7	DCIS	Residual DCIS	Benign breast tissue	2	2	15
14	HNB-LGMA 8	DCIS	No residual DCIS	Benign breast tissue	3	8	11
15	HNB-LGMA 9	DCIS	Residual DCIS	Benign breast tissue	1	1	17
HNB tissues with high-grade molecular abnormality (HNB-HGMA)
16	HNB-HGMA 1	Adenoid cystic carcinoma	No residual adenoid cystic carcinoma	Benign breast tissue	8	12	33
17	HNB-HGMA 2	DCIS	DCIS, multifocal	Benign breast tissue	12.5	20	64
18	HNB-HGMA 3	Intracystic carcinoma	No residual intracystic carcinoma	Benign breast tissue	12.5	8	35
19	HNB-HGMA 4	IDC	IDC focal papillary features	Benign breast tissue	9	30	113
20	HNB-HGMA 5	IDC	IDC	Benign breast tissue	2	12	46
21	HNB-HGMA 6	IDC	No residual IDC	Benign breast tissue	15	30	46
22	HNB-HGMA 7	No prior biosy performed	Benign breast tissue-patient BRCA1+, strong family history of BC	Benign breast tissue	10	25	106
23	HNB-HGMA 8	DCIS	No residual DCIS	Benign breast tissue	4	12	43
24	HNB-HGMA 9	IDC, ILC	No residual invasive carcinoma	Benign breast tissue	30	35	94