Research Article

Nanotherapeutics Using an HIV-1 Poly A and Transactivator of the HIV-1 LTR-(TAR-) Specific siRNA

Figure 4

The dose-dependent kinetics of QR-si510 siRNA nanoplex on HIV-1-infected THP-1 cells. THP-1 monocytic cultures were treated with lipofectamine transfected scrambled siRNA (10 nM), Lipofectamine transfected si510 HIV-1 siRNA (10 nM) and the QR-si510 siRNA (10 nM) nanoplex for time period ranging from 12 hr to 1 week posttransfection. At the end of the incubation period, the p24 production was measured in the supernatants using a commercially available p24 ELISA kit. Untransfected HIV-1-infected THP-1 and the non-HIV-1-infected THP-1 cells (nonvirus control) were the experimental controls. The concentration of both the scrambled siRNA and the si510 HIV-1 siRNA were 10 nM and the lipofectamine was used as per manufacturer’s protocol. Our results showed that 90% suppression of viral replication was observed 48 hr posttransfection and that further this suppression was observed even 1 week posttransfection. The results shown are mean ± SD of 3 separate experiments.
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