Endoscopic-Ultrasound-Guided Fine-Needle Aspiration and the Role of the Cytopathologist in Solid Pancreatic Lesion Diagnosis

Figure 4

Diff-Quik staining method. Air-dried smears are stained in three steps and takes about 10–20 seconds each. The smear is first fixed in methyl alcohol solution, followed by staining with solution I containing L-xanthene (an eosin variant), and then solution II containing methylene blue and azure A. The slide is then rinsed with water and the wet slide is viewed under the microscope.