Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis
Figure 5
Representative flow cytometric profiles of complement-independent cytotoxicity of 14F7 Mab in the murine myeloma derived cell line, P3X63Ag.653. (a) and (b): Dot plots graphs. Correlated measurements of FSC-H (cell-surface area or size) and SSC-H (cell granularity or internal complexity) permit to detect changes in the light scattering properties of cells. Observe: a homogeneous population of cells treated with P3 Mab (irrelevant control) (a). The treatment with 14F7 Mab altered the morphology of P3X63Ag.653 cells, characterized by changes in cell size and granularity based on both FSC-H and SSC-H parameters analysis (b). (c) and (d): Histogram graphs showing the discrimination of viable (M1) and nonviable (M2) cells using the propidium iodide (PI) staining. These graphs correspond to (a) and (b), respectively. Nonviable cells uptake this dye fluorescing brightly in the red range of the visible color spectrum (FL2-H). A limited fraction of P3X63Ag.653 cells showed a positive staining with PI after incubation with P3 Mab (M2 region). Note: the dramatic decreasing in the cell viability after treatment with 14F7 Mab.
