Review Article

Review of the History and Current Status of Cell-Transplant Approaches for the Management of Neuropathic Pain

Table 3

Strategies for creating cell lines.

Source Model ResultsAntinocicptive Molecule Released

Conditionally immortalized Cell lines:
Embryonic rat Raphe/SV40tsTag [102, 103, 275277]
Embryonic rat DRG neuron, 50B11 [90]
Human DRG neuron, HD10.6, v-myc [278]
Embryonic rat and bovine Chromaffin [138, 139]
Human embryonic Chromaffin cells/tsTag [279]
partial nerve injury (CCI) [139](i) Expressed capsaicin receptor transient receptor potential vanilloid family-1 (TRPV-1) and responded to capsaicin in vitro [90]
(ii) Expressed sensory neuron-associated transcription factors and exhibited capsaicin sensitivity [278]
(iii) Antinociceptive [139]
(i) enkephalin? [279, 280]

Bioengineered rat Raphe/tsTag/BDNF [110, 211]
Rat raphe/tsTag/galanin [111]
Rat raphe/tsTag/GAD67 [113]
Rat chromaffin/tsTag/Met-ENK [165, 170]
Partial nerve injury (CCI) [84, 110, 113, 158, 211, 229]
Formalin/c-fos induction [170]
Hemisection SCI [115118]
(i) Antinociceptive [84, 110, 113, 116, 119, 158, 211]
(ii) Restores dorsal horn GAD/GABA system in CCI [229]
(iii) Only intrathecal, not intraspinal, grafts of 5HT cells are antinociceptive [115]
(iv) Attenuates bilateral DH hypersensitivity [117]
(v) Restores spinal serotonin, downregulates the serotonin transporter, and increases BDNF tissue content [118]
(vi) Reduce formalin-evoked c-fos expression [170]
(i) 5HT [110, 116118, 211, 229]
(ii) BDNF [110, 118, 211]
(iii) GABA [110, 113]
(iv) galanin [111]
(v) Met-enkephalin [170]

Reversibly immortalized Cell Lines:
Tetracycline-regulated of SV40 large T-antigen (Tag) in human embryonic stem (ES) cells and mice [147]
Cre/lox-regulated Disimmortalizable Embryonic rat chromaffin cells [158]
Partial nerve injury (CCI) [158] (i) Antinociceptive [158](i) enkephalin? [158]
(ii) release norepinephrine [158]