Research Article

Preliminary Analysis of the Nonsynonymous Polymorphism rs17563 in BMP4 Gene in Brazilian Population Suggests Protection for Nonsyndromic Cleft Lip and Palate

Table 1

Primers, restriction enzyme, and fragment lengths for rs17563 of BMP4 gene.

SNPPrimerAnnealing temperatureRestriction enzymeFragment lengths

rs17563Forward 5′CACCATTCATTGCCCAAC3′56°CHphIT Allele: 195pb + 229pb
Reverse 5′AGTTTGGCTGCTTCTCCC3′C Allele: 424pb