]>Functional Morphology of Secretion by the Large Wax Glands (Sensilla Sagittiformia) Involved in Tick Defense : Table 1
Table 1: Number of days required for the large wax glands to replenish secretion by different nonfed stages of R. sanguineus (93% RH, 25°C) based on restimulation (leg pinching) following initial exhaustion of secretory reserves. L, larva; N, nymph; AM, adult male; AF adult female; dead tick, killed by freezing and then thawed to room temperature (shown); *, results from HCN-killed were similar. Data are mean  + SE and values followed by the same superscript letter within a column are not significantly different (ANOVA; 𝑃 < . 0 5 ). ( N = 3 replicates of 10 ticks for each time point with each replicate coming from a separate rearing batch of ticks).
% body mass lost after exhausting secretion
LNAMAF
Stimulated once
 Dead tick*0 000
 Live tick 1 . 2 5 ± 0 . 1 2 a 1 . 3 9 ± 0 . 2 4 a 2 . 5 1 ± 0 . 0 9 a 2 . 3 3 ± 0 . 1 7 a
Restimulation (day)
 00b0b0b0b
 2 0 . 2 4 ± 0 . 1 3 c 0 . 7 7 ± 0 . 0 9 c 0 . 3 9 ± 0 . 1 1 c 0 . 5 4 ± 0 . 1 8 c
 4 0 . 7 1 ± 0 . 0 7 d 1 . 0 2 ± 0 . 2 3 d 1 . 2 4 ± 0 . 0 8 d 0 . 9 2 ± 0 . 1 3 d
 6 0 . 8 2 ± 0 . 2 0 e 1 . 4 1 ± 0 . 1 4 a 1 . 1 6 ± 0 . 0 7 e 1 . 7 3 ± 0 . 1 6 e
 8 1 . 2 1 ± 0 . 0 8 a 1 . 3 2 ± 0 . 2 0 a 2 . 1 0 ± 0 . 1 2 f 1 . 6 7 ± 0 . 0 6 f
 10 1 . 3 0 ± 0 . 0 9 a 1 . 4 8 ± 0 . 1 2 a 2 . 4 4 ± 0 . 0 7 a 2 . 0 3 ± 0 . 1 9 g
 12 1 . 2 2 ± 0 . 1 7 a 1 . 4 1 ± 0 . 1 5 a 2 . 6 3 ± 0 . 0 9 a 2 . 2 9 ± 0 . 2 4 a
 14 1 . 3 6 ± 0 . 2 1 a 1 . 4 4 ± 0 . 0 9 a 2 . 5 8 ± 0 . 1 7 a 2 . 2 6 ± 0 . 1 1 a
Body size
 Initial mass (mg) 0 . 0 3 4 7 ± 0 . 0 0 3 0 . 1 4 7 ± 0 . 0 0 9 2 . 1 6 ± 0 . 5 4 3 . 3 2 ± 0 . 2 6