Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch: Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis
Figure 1
Chondrosarcomas acquire aberrant expression of epithelial-specific genes -cadherin and desmocollin-3. (a) E-cadherin mRNA expression was measured by real-time PCR. Four of the five chondrosarcoma cell lines (CS8E, CSPG, JJ, and CS13H) showed a significant increase in E-cadherin mRNA expression from approximately 6- to 189-fold increase when compared to the normal SNM83 chondrocyte cell line. SNM83 had minimal expression but was set at 1 on graph as calculated using CT relative quantification method. (b) Desmocollin 3 mRNA expression was measured by real-time PCR. Four of the five chondrosarcoma cell lines (CS8E, CSPG, JJ, and CS13H) showed a significant increase from approximately 1.5- to 12-fold more Desmocollin 3 mRNA when compared to normal line. Again SNM83 had minimal expression but was arbitrarily set at 1 for calculation purposes.
