598218.fig.001a
(a)
598218.fig.001b
(b)
Figure 1: Chondrosarcomas acquire aberrant expression of epithelial-specific genes 𝐸 -cadherin and desmocollin-3. (a) E-cadherin mRNA expression was measured by real-time PCR. Four of the five chondrosarcoma cell lines (CS8E, CSPG, JJ, and CS13H) showed a significant increase in E-cadherin mRNA expression from approximately 6- to 189-fold increase when compared to the normal SNM83 chondrocyte cell line. SNM83 had minimal expression but was set at 1 on graph as calculated using Δ Δ CT relative quantification method. (b) Desmocollin 3 mRNA expression was measured by real-time PCR. Four of the five chondrosarcoma cell lines (CS8E, CSPG, JJ, and CS13H) showed a significant increase from approximately 1.5- to 12-fold more Desmocollin 3 mRNA when compared to normal line. Again SNM83 had minimal expression but was arbitrarily set at 1 for calculation purposes.