598218.fig.002a
(a)
598218.fig.002b
(b)
Figure 2: Chondrosarcomas acquire aberrant expression of epithelial-specific genes maspin and 14-3-3σ. (a) Maspin mRNA expression was measured by real-time PCR. Four of the five chondrosarcoma cell lines (CSPG, JJ, NH69, and CS13H) showed a significant increase in maspin mRNA expression when compared to the normal SNM83 chondrocyte cell line. SNM83 had minimal expression but was set at 1 on graph as calculated using Δ Δ CT relative quantification method. (b) 14-3-3σ mRNA expression was measured by real-time PCR. Three of the five chondrosarcoma cell lines (JJ, NH69 and CS13H) showed a significant increase from approximately 50- to 150-fold more 14-3-3σ mRNA when compared to normal SNM83 and the CS8E and CSPG chondrosarcoma cell lines. Again SNM83 had minimal expression but was arbitrarily set at 1 for calculation purposes.