Figure 1: ALCAM/CD166 is highly expressed in osteosarcoma cell lines. Western immunoblot analysis of osteosarcoma cell lines probed with -ALCAM antisera. There is high total cell expression of ALCAM in HOS, KHOS, KHOS240s, and SJSA with moderate expression in the MNNG-HOS cell line. A pancreatic cancer cell line, HPAF, with known high levels of ALCAM expression was used as a positive control. MiaPaCa, a pancreatic cancer cell line with known lack of ALCAM expression served as a negative control. Membrane-localized ALCAM isoform is present at 105 kDa. β-Actin was used as an internal loading control.