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Figure 2: Surface ALCAM expression in osteosarcoma cell lines. Fluorescent immunohistochemistry showing membranous ALCAM expression in osteosarcoma cell lines in comparison to a known ALCAM expressing pancreatic cancer cell line, HPAF (positive control), and ALCAM negative cell line, MiaPaCa. ALCAM binding shown in red (Alexa Fluor 564) and nuclei counterstained with DAPI (20x magnification).